Recombinant Human Sonic Hedgehog is produced by our E.coli expression system and the target gene encoding Cys24-Gly197 is expressed.
Background
Sonic Hedgehog Homolog (SHH) belongs to a three-protein family called hedgehog. The other two family members are Indian Hedgehog (IHH) and Desert Hedgehog (DHH). Hedgehog proteins are key signaling molecules in embryonic development. SHH is expressed in various embryonic tissues and plays critical roles in regulating the patterning of many systems, such as limbs and brain. SHH also plays an important role in adult, including the division of adult stem cells and the development of certain cancers and other diseases. Human SHH is expressed as a 45kDa precursor, and undergoes a series of processing during secretion. After the removal of the signal peptide, a protease within the C-terminal domain catalyzes the cleavage of SHH into a 20 kDa N-terminal signaling domain (SHH-N) and a 25 kDa C-terminal domain (SHH-C). SHH-N has the “all signaling” capability. SHH-N binds to the 12 pass transmembrane protein Patched (Ptc) on cell surface, which releases the repression of the activity of Smoothened (Smo), a G-protein coupled receptor, by Ptc.
Reconstitution
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100 μg/ml.Dissolve the lyophilized protein in ddH2O.Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Storage
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at < -20°C for 3 months
關鍵字： Recombinant Human Sonic Hedgehog/Shh;細胞生物學
北京索萊寶科技有限公司是一家集研發(fā)、生產(chǎn)、銷售、服務于一體的高科技生物企業(yè)。技術推廣;技術轉讓；技術咨詢；技術服務；生物試劑技術開發(fā)；銷售生物試劑盒、實驗室專用化學試劑、實驗室專用設備、辦公用品、化工產(chǎn)品（不含危險化學品）、計算機軟件及輔助設備；貨物進出口、技術進出口、代理進出口；委托加工生產(chǎn)生物制品、生化制品、化工產(chǎn)品（不含危險化學品）；生產(chǎn)生物試劑盒；批發(fā)第一類醫(yī)療器械、第二類醫(yī)療器械；批發(fā)第三類醫(yī)療器械。