| 產(chǎn)品編號(hào) |
產(chǎn)品名稱 |
產(chǎn)品包裝 |
產(chǎn)品價(jià)格 |
| AF1666 |
Calreticulin Rabbit Monoclonal Antibody |
50μl |
618.00元 |
|
來(lái)源
|
用途
|
交叉反應(yīng)性
|
分子量
|
|
Rabbit
|
WB, IP, IF, IHC, ICC, FC
|
H, M, R
|
60.0kDa
|
???????WB, Western blot; IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry; ICC,
Immunocytochemistry; FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent Assay; ChIP, Chromatin
Immunoprecipitation Assay.
???????H, Human; M, Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr, Horse; Mk,
Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, Zebrafish; All, all species expected.
???????配套提供了Western一抗稀釋液，可以用于Western檢測(cè)或其它適當(dāng)用途時(shí)的一抗稀釋。
???????建議抗體使用時(shí)的稀釋比例如下(實(shí)際使用時(shí)需根據(jù)抗原水平的高低作適當(dāng)調(diào)整)：
|
WB
|
IP
|
IF
|
IHC
|
ICC
|
FC
|
ELISA
|
ChIP
|
|
1:1,000-1:5,000
|
1:10-1:50
|
1:100-1:500
|
1:50-1:200
|
1:100-1:500
|
1:50-1:100
|
-
|
-
|
抗體詳細(xì)信息如下:
|
About this Antibody
|
|
Name
|
Calreticulin Rabbit Monoclonal Antibody
|
|
Category
|
Rabbit Monoclonal Antibody (RabMAb); Primary antibody
|
|
Isotype
|
IgG
|
|
Purification
|
Affinity purification
|
|
About the Immunogen
|
|
Immunogen
|
Recombinant protein
|
|
Gene ID
|
811(Human); 12317(Mouse); 64202(Rat)
|
|
SwissProt
|
P27797(Human); P14211(Mouse); P18418(Rat)
|
|
Synonyms
|
CRT; HEL-S-99n; RO; SSA; cC1qR; CALR
|
|
Category
|
Others
|
|
Background
|
Calnexin and calregulin (also called calreticulin) are calcium-binding proteins that are localized to the endoplasmic reticulum, Calnexin to the membrane and calregulin to the lumen. Calnexin is a type I membrane protein that interacts with newly synthesized glycoproteins in the endoplasmic reticulum. It may play a role in assisting with protein assembly and in retaining unassembled protein subunits in the endoplasmic reticulum. Calregulin has both low- and high-affinity calcium-binding sites. Neither Calnexin nor calregulin contains the calcium-binding "E-F hand" motif found in calmodulins. Calnexin and calregulin are important for the maturation of glycoproteins in the endoplasmic reticulum and appear to bind many of the same proteins.
|
包裝清單:
|
產(chǎn)品編號(hào)
|
產(chǎn)品名稱
|
包裝
|
|
AF1666
|
Calreticulin Rabbit Monoclonal Antibody
|
50μl
|
|
AZ050
|
Western一抗稀釋液
|
50ml
|
|
-
|
說(shuō)明書
|
1份
|
保存條件:
???????Calreticulin Rabbit Monoclonal Antibody -20℃保存，Western一抗稀釋液-20℃或4℃保存，一年有效。Western一抗稀釋液優(yōu)先推薦4℃保存，長(zhǎng)期不使用可以考慮-20℃保存，但凍融可能會(huì)導(dǎo)致出現(xiàn)輕微的渾濁和少量不溶物。
注意事項(xiàng):
???????如果本抗體用于Western blot (WB)、免疫熒光(IF)、免疫細(xì)胞化學(xué)(ICC)等實(shí)驗(yàn)，請(qǐng)注意回收使用過(guò)的稀釋抗體?；厥盏目贵w通常至少可以重復(fù)使用5-10次。稀釋后的抗體，包括已經(jīng)使用過(guò)的稀釋抗體，請(qǐng)4℃保存。
???????回收后重復(fù)使用的抗體，使用方法同新鮮稀釋的抗體。如果在重復(fù)使用過(guò)程中發(fā)現(xiàn)抗體出現(xiàn)輕微混濁現(xiàn)象，可以10,000g離心1-3分鐘，取上清用于后續(xù)檢測(cè)。如果回收的抗體出現(xiàn)明顯的絮狀物或長(zhǎng)霉長(zhǎng)菌等情況，則可以考慮廢棄該抗體。
???????提供的Western一抗稀釋液也可以用于免疫熒光(IF)、免疫組化(IHC)、免疫細(xì)胞化學(xué)(ICC)等適當(dāng)用途。如果希望獲得的檢測(cè)效果，請(qǐng)考慮使用上述檢測(cè)專用的一抗稀釋液。
???????本產(chǎn)品僅限于專業(yè)人員的科學(xué)研究用，不得用于臨床診斷或治療，不得用于食品或藥品，不得存放于普通住宅內(nèi)。
???????為了您的安全和健康，請(qǐng)穿實(shí)驗(yàn)服并戴一次性手套操作。