產品編號 |
產品名稱 |
產品包裝 |
產品價格 |
AF1072 |
NeuN Rabbit Monoclonal Antibody |
50μl |
618.00元 |
來源
|
用途
|
交叉反應性
|
分子量
|
Rabbit
|
WB, IP, IF, IHC, ICC, FC
|
H, M, R
|
34.0kDa
|
???????WB, Western blot; IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry; ICC,
Immunocytochemistry; FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent Assay; ChIP, Chromatin
Immunoprecipitation Assay.
???????H, Human; M, Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr, Horse; Mk,
Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, Zebrafish; All, all species expected.
???????配套提供了Western一抗稀釋液,可以用于Western檢測或其它適當用途時的一抗稀釋。
???????建議抗體使用時的稀釋比例如下(實際使用時需根據(jù)抗原水平的高低作適當調整):
WB
|
IP
|
IF
|
IHC
|
ICC
|
FC
|
ELISA
|
ChIP
|
1:1000-1:2000
|
1:50-1:200
|
1:50-1:200
|
1:200-1:500
|
1:50-1:200
|
1:50-1:100
|
-
|
-
|
抗體詳細信息如下:
About this Antibody
|
Name
|
NeuN Rabbit Monoclonal Antibody
|
Category
|
Rabbit Monoclonal Antibody (RabMAb); Primary antibody
|
Isotype
|
IgG
|
Purification
|
Affinity purification
|
About the Immunogen
|
Immunogen
|
Recombinant protein
|
Gene ID
|
146713(Human); 52897(Mouse)
|
SwissProt
|
A6NFN3(Human); Q8BIF2(Mouse)
|
Synonyms
|
FOX3; NEUN; FOX-3; HRNBP3
|
Category
|
Epigenetics; Neuronal Signaling
|
Background
|
Neuronal nuclei (NeuN, Fox-3, RBFOX3) is a nuclear protein expressed in most post-mitotic neurons of the central and peripheral nervous systems. NeuN is not detected in Purkinje cells, sympathetic ganglion cells, Cajal-Retzius cells, INL retinal cells, inferior olivary, and dentate nucleus neurons. This neuronal protein was originally identified by immunoreactivity with a monoclonal antibody also called NeuN. Using MS-analysis, NeuN was later identified as the Fox-3 gene product. Fox-3 contains an RNA recognition motif and functions as a splicing regulator. Fox-3 regulates alternative splicing of NumB, promoting neuronal differentiation during development.
|
包裝清單:
產品編號
|
產品名稱
|
包裝
|
AF1072
|
NeuN Rabbit Monoclonal Antibody
|
50μl
|
AZ050
|
Western一抗稀釋液
|
50ml
|
-
|
說明書
|
1份
|
保存條件:
???????NeuN Rabbit Monoclonal Antibody -20℃保存,Western一抗稀釋液-20℃或4℃保存,一年有效。Western一抗稀釋液優(yōu)先推薦4℃保存,長期不使用可以考慮-20℃保存,但凍融可能會導致出現(xiàn)輕微的渾濁和少量不溶物。
注意事項:
???????如果本抗體用于Western?blot?(WB)、免疫熒光(IF)、免疫細胞化學(ICC)等實驗,請注意回收使用過的稀釋抗體?;厥盏目贵w通常至少可以重復使用5-10次。稀釋后的抗體,包括已經使用過的稀釋抗體,請4℃保存。
???????回收后重復使用的抗體,使用方法同新鮮稀釋的抗體。如果在重復使用過程中發(fā)現(xiàn)抗體出現(xiàn)輕微混濁現(xiàn)象,可以10,000g離心1-3分鐘,取上清用于后續(xù)檢測。如果回收的抗體出現(xiàn)明顯的絮狀物或長霉長菌等情況,則可以考慮廢棄該抗體。
???????提供的Western一抗稀釋液也可以用于免疫熒光(IF)、免疫組化(IHC)、免疫細胞化學(ICC)等適當用途。如果希望獲得的檢測效果,請考慮使用上述檢測專用的一抗稀釋液。
???????本產品僅限于專業(yè)人員的科學研究用,不得用于臨床診斷或治療,不得用于食品或藥品,不得存放于普通住宅內。
???????為了您的安全和健康,請穿實驗服并戴一次性手套操作。