| 產(chǎn)品編號(hào) |
產(chǎn)品名稱(chēng) |
產(chǎn)品包裝 |
產(chǎn)品價(jià)格 |
| AF1936 |
Prion Protein (PrP) Rabbit Monoclonal Antibody |
50μl |
618.00元 |
|
來(lái)源
|
用途
|
交叉反應(yīng)性
|
分子量
|
|
Rabbit
|
WB, IF, IHC, ICC, FC
|
H, M, R
|
28.0kDa
|
???????WB, Western blot; IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry; ICC,
Immunocytochemistry; FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent Assay; ChIP, Chromatin
Immunoprecipitation Assay.
???????H, Human; M, Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr, Horse; Mk,
Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, Zebrafish; All, all species expected.
???????配套提供了Western一抗稀釋液，可以用于Western檢測(cè)或其它適當(dāng)用途時(shí)的一抗稀釋。
???????建議抗體使用時(shí)的稀釋比例如下(實(shí)際使用時(shí)需根據(jù)抗原水平的高低作適當(dāng)調(diào)整)：
|
WB
|
IP
|
IF
|
IHC
|
ICC
|
FC
|
ELISA
|
ChIP
|
|
1:1,000-1:5,000
|
-
|
1:50-1:200
|
1:50-1:200
|
1:50-1:200
|
1:50-1:100
|
-
|
-
|
抗體詳細(xì)信息如下:
|
About this Antibody
|
|
Name
|
Prion Protein (PrP) Rabbit Monoclonal Antibody
|
|
Category
|
Rabbit Monoclonal Antibody (RabMAb); Primary antibody
|
|
Isotype
|
IgG
|
|
Purification
|
Affinity purification
|
|
About the Immunogen
|
|
Immunogen
|
Recombinant protein
|
|
Gene ID
|
5621(Human); 19122(Mouse); 24686(Rat)
|
|
SwissProt
|
P04156(Human); P04925(Mouse); P13852(Rat)
|
|
Synonyms
|
ASCR; AltPrP; CD230; CJD; GSS; KURU; PRIP; PrP; PrP27-30; PrP33-35C; PrPc; p27-30
|
|
Category
|
Neuronal Signaling; Stem Cells & Wnt
|
|
Background
|
Prion diseases, or transmissible spongiform encephalopathies (TSEs), are manifested as genetic, infectious or sporadic, lethal neurodegenerative disorders involving alterations of the prion protein (PrP). Characteristic of prion diseases, cellular PrP (PrPc) is converted to the disease form, PrPSc, through alterations in the protein folding conformations. PrPc is constitutively expressed in normal adult brain and is sensitive to proteinase K digestion, while the altered PrPSc conformation is resistant to proteases, resulting in a distinct molecular mass after PK treatment. Consistent with the transient infection process of prion diseases, incubation of PrPc with PrPSc both in vitro and in vivo produces PrPc that is resistant to protease degradation. Infectious PrPSc is found at high levels in the brains of animals affected by TSEs, including scrapie in sheep, BSE in cattle and Cruetzfeldt-Jakob disease in humans.
|
包裝清單:
|
產(chǎn)品編號(hào)
|
產(chǎn)品名稱(chēng)
|
包裝
|
|
AF1936
|
Prion Protein (PrP) Rabbit Monoclonal Antibody
|
50μl
|
|
AZ050
|
Western一抗稀釋液
|
50ml
|
|
-
|
說(shuō)明書(shū)
|
1份
|
保存條件:
???????Prion Protein (PrP) Rabbit Monoclonal Antibody -20℃保存，Western一抗稀釋液-20℃或4℃保存，一年有效。Western一抗稀釋液優(yōu)先推薦4℃保存，長(zhǎng)期不使用可以考慮-20℃保存，但凍融可能會(huì)導(dǎo)致出現(xiàn)輕微的渾濁和少量不溶物。
注意事項(xiàng):
???????如果本抗體用于Western blot (WB)、免疫熒光(IF)、免疫細(xì)胞化學(xué)(ICC)等實(shí)驗(yàn)，請(qǐng)注意回收使用過(guò)的稀釋抗體?；厥盏目贵w通常至少可以重復(fù)使用5-10次。稀釋后的抗體，包括已經(jīng)使用過(guò)的稀釋抗體，請(qǐng)4℃保存。
???????回收后重復(fù)使用的抗體，使用方法同新鮮稀釋的抗體。如果在重復(fù)使用過(guò)程中發(fā)現(xiàn)抗體出現(xiàn)輕微混濁現(xiàn)象，可以10,000g離心1-3分鐘，取上清用于后續(xù)檢測(cè)。如果回收的抗體出現(xiàn)明顯的絮狀物或長(zhǎng)霉長(zhǎng)菌等情況，則可以考慮廢棄該抗體。
???????提供的Western一抗稀釋液也可以用于免疫熒光(IF)、免疫組化(IHC)、免疫細(xì)胞化學(xué)(ICC)等適當(dāng)用途。如果希望獲得的檢測(cè)效果，請(qǐng)考慮使用上述檢測(cè)專(zhuān)用的一抗稀釋液。
???????本產(chǎn)品僅限于專(zhuān)業(yè)人員的科學(xué)研究用，不得用于臨床診斷或治療，不得用于食品或藥品，不得存放于普通住宅內(nèi)。
???????為了您的安全和健康，請(qǐng)穿實(shí)驗(yàn)服并戴一次性手套操作。