價(jià)格 | 詢價(jià) |
包裝 | 50μg |
最小起訂量 | 50μg |
發(fā)貨地 | 上海 |
更新日期 | 2024-12-25 |
中文名稱:Recombinant eGFP | 英文名稱:Recombinant eGFP |
有效期: 一年 | 產(chǎn)品類別: 重組蛋白 其他重組蛋白 |
產(chǎn)品編號(hào) | 產(chǎn)品名稱 | 產(chǎn)品包裝 | 產(chǎn)品價(jià)格 |
P7410-50μg | Recombinant eGFP | 50μg | 1159.00元 |
Species | Gene ID | Accession | Source | Length | MW | Tag |
- | 7011691 | B6UPG7 | E. coli | 239aa | 26.9kDa | - |
About this protein | |
Name | Recombinant eGFP (Recombinant Enhanced Green Fluorescent Protein; reGFP);重組增強(qiáng)綠色熒光蛋白 |
Synonyms | eGFP; GFPuv; Enhanced Green Fluorescent Protein |
Purity | >95% by SDS-PAGE and HPLC analyses. |
Biological Activity | N/A |
Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
Formulation | Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH7.4. |
Endotoxin | Less than 1EU/μg of reGFP as determined by LAL method. |
Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions. |
Category | Others |
Background | Green fluorescent protein (GFP) here refers to the protein first purified from jellyfish Aequorea victoria, though many other organisms have similar proteins. It is a 26.9kDa protein (composed of 238a.a. residues) that shows green fluorescence in shortwave light (blue to ultraviolet). Despite of wild-type GFP, many mutants of GFP have been engineered for wider usage in research. Enhanced GFP (eGFP) has S65T and F64L mutations, which make GFP show increased fluorescence and fold more efficiently under 37℃, respectively. eGFP allows the use of GFP in mammalian cells. In A. Victoria, GFP plays roles as an energy transfer acceptor. It has long been used in cell and molecular biology as a reporter of gene expression. GFP can also been applied as a molecular thermometer to measure temperature accurately in fluids. |
Amino Acid Sequence | MVSKGEELFT GVVPILVELD GDVNGHKFSV SGEGEGDATY GKLTLKFICT TGKLPVPWPT LVTTLTYGVQ CFSRYPDHMK QHDFFKSAMP EGYVQERTIF FKDDGNYKTR AEVKFEGDTL VNRIELKGID FKEDGNILGH KLEYNYNSHN VYIMADKQKN GIKVNFKIRH NIEDGSVQLA DHYQQNTPIG DGPVLLPDNH YLSTQSALSK DPNEKRDHMV LLEFVTAAGI TLGMDELYK |
包裝清單:
產(chǎn)品編號(hào) | 產(chǎn)品名稱 | 包裝 |
P7410-10μg | Recombinant eGFP | 10μg |
P7410-50μg | Recombinant eGFP | 50μg |
P7410-1mg | Recombinant eGFP | 1mg |
- | 說(shuō)明書(shū) | 1份 |
保存條件:
???????-20℃或更低溫度保存,至少一年有效。由于蛋白的每次凍融均會(huì)引起部分失活,所以首次配制成相應(yīng)濃度的儲(chǔ)存液后(請(qǐng)根據(jù)產(chǎn)品簡(jiǎn)介中Reconstitution一欄的信息配制儲(chǔ)存液),須分裝后-20℃或更低溫度凍存,以避免反復(fù)凍融。
注意事項(xiàng):
???????由于有些塑料管壁對(duì)某些蛋白有較強(qiáng)的吸附作用,溶液中的蛋白很容易粘附在管壁上,并且粘附后的蛋白很難與管壁分離。而載體蛋白(Carrier protein,如0.1% BSA等)的主要作用是預(yù)先封閉塑料管壁上的蛋白結(jié)合位點(diǎn),使細(xì)胞因子或重組蛋白不會(huì)粘附于管壁。所以一定要使用產(chǎn)品簡(jiǎn)介中Reconstitution一欄的信息配制儲(chǔ)存液。
???????本產(chǎn)品僅限于專業(yè)人員的科學(xué)研究用,不得用于臨床診斷或治療,不得用于食品或藥品,不得存放于普通住宅內(nèi)。
???????為了您的安全和健康,請(qǐng)穿實(shí)驗(yàn)服并戴一次性手套操作。
成立日期 | 2007-07-19 (18年) | 注冊(cè)資本 | 1000.000000萬(wàn)人民幣 |
員工人數(shù) | 500人以上 | 年?duì)I業(yè)額 | ¥ 500萬(wàn)-1000萬(wàn) |
主營(yíng)行業(yè) | 生化試劑,核苷,核苷酸,寡核苷酸,抗體,蛋白組學(xué),生物活性小分子 | 經(jīng)營(yíng)模式 | 試劑 |
產(chǎn)品名稱 | 價(jià)格 | 公司名稱 | 報(bào)價(jià)日期 | |
---|---|---|---|---|
詢價(jià) |
VIP4年
|
杭州固拓生物科技有限公司
|
2024-12-26 | |
¥960.00 |
VIP9年
|
北京索萊寶科技有限公司
|
2024-12-26 | |
¥888 |
VIP6年
|
上海康朗生物科技有限公司
|
2024-12-26 |