Species

Gene ID

Accession

Source

Length

MW

Tag

Rat

24628

Q05028

E. coli

109aa

24.4kDa

－

About this Antibody

Name

Recombinant Rat PDGF-BB (Recombinant Rat Platelet-derived Growth Factor-BB; rRtPDGF-BB)；重組大鼠血小板衍生生長因子-BB

Synonyms

Platelet-derived growth factor subunit B; PDGF subunit B; PDGF-2; Platelet-derived growth factor B chain; Platelet-derived growth factor beta polypeptide; Pdgfb

Purity

>98% by SDS-PAGE and HPLC analyses.

Biological Activity

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 2.0ng/ml, corresponding to a specific activity of >5.0×10⁵IU/mg.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a 0.2μm filtered concentrated solution in 1X PBS, pH6.9.

Endotoxin

Less than 0.1EU/μg of rRtPDGF-BB as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.

Category

Cytokine

Background

Platelet-derived growth factor is a dimeric glycoprotein composed of two A (-AA) or two B (-BB) chains or a combination of the two (-AB). All PDGFs function as secreted, disulphide-linked homodimers, but only PDGFA and B can form functional heterodimers. PDGFs are mitogenic during early developmental stages, driving the proliferation of undifferentiated mesenchyme and some progenitor populations. PDGF is a required element in cellular division for fibroblasts, a type of connective tissue cell that is especially prevalent in wound healing. In essence, the PDGFs allow a cell to skip the G1 checkpoints in order to divide. Recombinant rat PDGF-AA contains 109 amino acids and has a molecular mass of 12.2kDa.

Amino Acid Sequence

SLGSLAAAEP AVIAECKTRT EVFQISRNLI DRTNANFLVW PPCVEVQRCS GCCNNRNVQC RASQVQMRPV QVRKIEIVRK KPVFKKATVT LEDHLACKCE TVVTPRPVT

產品編號

產品名稱

包裝

P6389-2μg

Recombinant Rat PDGF-BB

2μg

P6389-10μg

Recombinant Rat PDGF-BB

10μg

P6389-100μg

Recombinant Rat PDGF-BB

100μg

P6389-1mg

Recombinant Rat PDGF-BB

1mg

-

說明書

1份