Species

Gene ID

Accession

Source

Length

MW

Tag

Rat

60427

P21581

E. coli

165aa

18.5kDa

－

About this Antibody

Name

Recombinant Rat SCF (Recombinant Rat Stem Cell Factor; rRtSCF)；重組大鼠干細胞生長因子

Synonyms

c-kit Ligand; KITLG; MGF; SLF; DKFZp686F2250; familial progressive hyperpigmentation 2; FPH2; KIT ligand; Kitl; KL-1; Mast cell growth factor; MGFSHEP7; SCFStem cell factor; SFc-Kit ligand; steel factor

Purity

>95% by SDS-PAGE and HPLC analyses.

Biological Activity

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine MC/9-2 mast cells is less than 5ng/ml, corresponding to a specific activity of >2.0×10⁵IU/mg.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH7.4.

Endotoxin

Less than 1EU/μg of rRtSCF as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.

Category

Cytokine

Background

Stem Cell Factor (SCF) which binds to the c-Kit receptor is produced by fibroblasts and endothelial cells. The soluble and transmembrane forms of the protein are formed by alternative splicing of the same RNA transcript and the presence of both soluble and transmembrane SCF is required for normal hematopoietic function. SCF plays an important role in hematopoiesis, spermatogenesis, and melanogenesis. In addition, it also promotes mast cell adhesion, migration, proliferation, and survival 3. Rat SCF shares 75%-90%a.a. sequence identity with canine, feline, mouse, and human SCF. Furthermore, rat SCF is active on mouse and human cells, but human SCF is only weakly active on mouse cells.

Amino Acid Sequence

MQEICRNPVT DNVKDITKLV ANLPNDYMIT LNYVAGMDVL PSHCWLRDMV THLSVSLTTL LDKFSNISEG LSNYSIIDKL GKIVDDLVAC MEENAPKNVK ESLKKPETRN FTPEEFFSIF NRSIDAFKDF MVASDTSDCV LSSTLGPEKD SRVSVTKPFM LPPVA

產(chǎn)品編號

產(chǎn)品名稱

包裝

P6321-2μg

Recombinant Rat SCF

2μg

P6321-10μg

Recombinant Rat SCF

10μg

P6321-100μg

Recombinant Rat SCF

100μg

P6321-1mg

Recombinant Rat SCF

1mg

-

說明書

1份