Species

Gene ID

Accession

Source

Length

MW

Tag

Rat

83785

P16612

E. coli

165aa

38.7kDa

－

About this Antibody

Name

Recombinant Rat VEGF164 (Recombinant Rat Vascular Endothelial Growth Factor164; rRtVEGF164)；重組大鼠血管內(nèi)皮生長因子164

Synonyms

VAS; Vasculotropin; VEGFA; VPF; MVCD1; vascular endothelial growth factor A; Vascular permeability factor; VEGF-A; VEGFMGC70609; VPFvascular endothelial growth factor

Purity

>95% by SDS-PAGE and HPLC analyses.

Biological Activity

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human umbilical vein endothelial cells(HUVEC) is less than 5ng/ml, corresponding to a specific activity of >2.0×10⁵IU/mg.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a 0.2μm filtered concentrated solution in 20mM Tris, 300mM NaCl, pH8.8.

Endotoxin

Less than 1EU/μg of rRtVEGF164 as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.

Category

Cytokine

Background

Vascular Endothelial Growth Factor is a sub-family of growth factors produced by cells, which stimulates vasculogenesis and angiogenesis. VEGF's normal function is to create new blood vessels during embryonic development, new blood vessels after injury, muscle following exercise, and new vessels (collateral circulation) to bypass blocked vessels. Mouse and rat express alternately spliced isoforms of 120, 164, and 188 amino acids (a.a.) in length. Recombinant Rat VEGF164 contains 165 amino acids residues and it is a disulfide-linked homodimer. In addition, it shares 97%a.a. sequence identity with corresponding regions of mouse, 88% with human and bovine, 89% with porcine and canine, and 90% with feline and equine VEGF, respectively.

Amino Acid Sequence

MAPTTEGEQK AHEVVKFMDV YQRSYCRPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCAGC CNDEALECVP TSESNVTMQI MRIKPHQSQH IGEMSFLQHS RCECRPKKDR TKPEKHCEPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR

產(chǎn)品編號(hào)

產(chǎn)品名稱

包裝

P6380-2μg

Recombinant Rat VEGF164

2μg

P6380-10μg

Recombinant Rat VEGF164

10μg

P6380-100μg

Recombinant Rat VEGF164

100μg

P6380-1mg

Recombinant Rat VEGF164

1mg

-

說明書

1份