Species

Gene ID

Accession

Source

Length

MW

Tag

Tobacco

1502321

P04517

E. coli

－

－

－

About this Protein

Name

Recombinant Tobacco EV (Recombinant Tobacco Etch Virus Protease; rTEV)；重組煙草蝕紋病毒蛋白酶

Synonyms

polyprotein; 6K2 protein; NIa－VPg protein; NIb protein; coat protein; P1 protein; HC－Pro protein; P3 protein; 6K1 protein; CI protein; NIa－Pro protein

Purity

>90% by SDS-PAGE analysis.

Biological Activity

N/A

Physical Appearance

Clear colorless liquid.

Formulation

A 0.2μm filtered solution in 25mM Tris－HCl, pH8.0, 75mM NaCl, 5mM EDTA, 10mM GSH, with 50% Glycerol.

Endotoxin

N/A

Reconstitution

A number of variables can be changed to optimize the cleavage of any specific protein. The amount of rTEV, the temperature of the incubation, and the time needed for cleavage may be examined. If the protein of interest is heat－labile, then 4℃ incubations are recommended. Reactions at 4℃ will require longerincubation times and/or more rTEV.

Category

Enzymes

Background

TEV protease encoded by the tobacco etch virus is a catalytic domain of the Nuclear Inclusion a (NIa) protein. It is consists of 241a.a. with the molecular weight of 27kDa. TEV recognizes the amino acid sequence of the general form E-X-X-Y-X-Q (or S)/X', and cleaves between Q (or S)/X'. In this form X and X' stand for any of the amino acid residues, except that X' cannot be P. The optimal cleavage site is ENLYFQ/G. As having the absolute specificity and wildly using conditions like broad pH range and ionic strength, the TEV protease became more versatile than EK, thrombin and other protease used in biochemical applications, especially recombinant protein production. The optimal temperature for cleavage is 30℃; however, the enzyme can be used at temperatures as low as 4℃. Following digestion, TEV Protease can be removed from the reaction via the GST tag sequence by affinity chromatography.

Amino Acid Sequence

N/A

產(chǎn)品編號(hào)

產(chǎn)品名稱

包裝

P7068-300IU

Recombinant Tobacco EV

300IU

P7068-1000IU

Recombinant Tobacco EV

1000IU

P7068-10000IU

Recombinant Tobacco EV

10000IU

-

說(shuō)明書

1份