描述

Thermo Scientific PageBlue Protein Staining Solution is a sensitive, economical formulation of colloidal coomassie G-250 dye for endpoint staining of proteins in polyacrylamide gels and on PVDF membranes without methanol or acetic acid.

Features of PageBlue Protein Staining Solution:

• Easy-to-use—simply soak the gel in the ready-to-use solution and observe stained bands without destaining
• Fast—25 to 40 minute protocol
• Safe—does not contain methanol or acetic acid
• Dynamic range—linear dynamic range of 5 to 500ng
• Economical—can be reused up to 3 times

The simple PageBlue Protein Staining Solution protocol is sensitive, efficient and eliminates the concern of over staining gels even after overnight staining. This protein stain delivers a dynamic range of 5 to 500ng, which is approximately 10 times more sensitive than traditional coomassie R-250-based dyes. Furthermore, PageBlue Protein Staining Solution is economical because it can be reused up to three times without any loss in sensitivity.

Applications:
• Visualize and quantify proteins separated in 1D, 2D and IEF polyacrylamide gels
• Stain proteins after transfer onto PVDF membranes
• Stain gels prior to subsequent silver staining or mass spectrometry analysis