"SW620/LUC人結(jié)腸癌細(xì)胞代次低|培養(yǎng)基|送STR圖譜<熒光素酶標(biāo)記>

傳代比例：1:2-1:4(首次傳代建議1:2)

生長特性：貼壁生長

【細(xì)胞培養(yǎng)經(jīng)驗(yàn)分享】啟蒙老師的重要性：一般進(jìn)實(shí)驗(yàn)室都有師兄師姐帶著做，他們就是你做細(xì)胞的啟蒙老師。他們的操作手法、細(xì)節(jié)、理論講解就成了你操作的準(zhǔn)則，如營養(yǎng)液、細(xì)胞瓶的擺放位置、滅菌處理程序、開蓋手法、細(xì)胞吹打手法等等。要學(xué)會(huì)他們的正確操作，在第一次的時(shí)候就要重視。像養(yǎng)孩子一樣養(yǎng)細(xì)胞，細(xì)胞有時(shí)真的很脆弱，最好每天都去看看它，以防止出現(xiàn)培養(yǎng)箱缺水、缺二氧化碳、停電、溫度不夠等異?，F(xiàn)象，也好及時(shí)解決這些意外，避免重復(fù)實(shí)驗(yàn)帶來的更大痛苦。好細(xì)胞要及時(shí)保種：細(xì)胞要分批傳代，這樣即使有一批出了問題，還有一批備用的。像后者一般人可能不容易做到。但這是我血的教訓(xùn)，有一次細(xì)胞污染了，全軍覆沒。當(dāng)時(shí)可后悔沒有保種。細(xì)胞跟人一樣，不同的細(xì)胞，培養(yǎng)特性是不一樣的。培養(yǎng)過程中要細(xì)細(xì)體會(huì)，不同細(xì)胞系使用不同的培養(yǎng)基和血清。

換液周期：每周2-3次

U266 Bl Cells;背景說明：詳見相關(guān)文獻(xiàn)介紹;傳代方法：１：３傳代,２-３天傳一代;生長特性：懸浮生長 ;形態(tài)特性：淋巴母細(xì)胞樣;相關(guān)產(chǎn)品有：H1651細(xì)胞、TM4細(xì)胞、SU.86.86細(xì)胞

SPCA-1 Cells;背景說明：詳見相關(guān)文獻(xiàn)介紹;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁或懸浮，詳見產(chǎn)品說明書部分;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：Hs-688A-T細(xì)胞、BC-020細(xì)胞、CAL62細(xì)胞

Hs940-T Cells;背景說明：詳見相關(guān)文獻(xiàn)介紹;傳代方法：１：２-１：３傳代，２-３天換液１次。;生長特性：貼壁生長;形態(tài)特性：成纖維細(xì)胞;相關(guān)產(chǎn)品有：NCI-H2228細(xì)胞、SNU-886細(xì)胞、DMS 79細(xì)胞

SW620/LUC人結(jié)腸癌細(xì)胞代次低|培養(yǎng)基|送STR圖譜<熒光素酶標(biāo)記>

背景信息：詳見相關(guān)文獻(xiàn)介紹

┈訂┈購(技術(shù)服務(wù))┈熱┈線：1┈3┈6┈4┈1┈9┈3┈0┈7┈9┈1【微信同號(hào)】┈Q┈Q：3┈1┈8┈0┈8┈0┈7┈3┈2┈4；

貼壁細(xì)胞的傳代培養(yǎng)，詳細(xì)步驟如下：首先倒掉培養(yǎng)基，在這一步驟可以收集一些細(xì)胞上清做支原體檢測；加入胰蛋白酶，一般T25是加2mL，蓋好瓶蓋，搖晃T25培養(yǎng)瓶，使胰蛋白酶均勻覆蓋在細(xì)胞表面，放入培養(yǎng)箱2-3min，期間可在顯微鏡下觀察，看到大部分細(xì)胞變圓，即可放入超凈臺(tái)，加入2倍的完全培養(yǎng)基，這里就是加4mL培養(yǎng)基，終止消化；將含有胰蛋白酶，細(xì)胞和培養(yǎng)基一起轉(zhuǎn)移到離心管中，1000rpm/3min離心，去掉上清；新鮮的完全培養(yǎng)基重懸，根據(jù)細(xì)胞的生長特性和后續(xù)的實(shí)驗(yàn)需求進(jìn)行傳代，比如我養(yǎng)的Hepa1-6就長的比較快，不是著急用的話，我就會(huì)按1E6個(gè)細(xì)胞/T75培養(yǎng)瓶進(jìn)行傳代；但如果后兩天要用，就會(huì)適當(dāng)多傳一點(diǎn)；還可通過顯微鏡計(jì)數(shù)后，直接用于細(xì)胞鋪板，繼續(xù)后續(xù)的實(shí)驗(yàn)。

產(chǎn)品包裝：復(fù)蘇發(fā)貨：T25培養(yǎng)瓶(一瓶)或凍存發(fā)貨：1ml凍存管(兩支)

來源說明：細(xì)胞主要來源ATCC、ECACC、DSMZ、RIKEN等細(xì)胞庫

SW620/LUC人結(jié)腸癌細(xì)胞代次低|培養(yǎng)基|送STR圖譜<熒光素酶標(biāo)記>

物種來源：人源、鼠源等其它物種來源

DHL-6 Cells;背景說明：詳見相關(guān)文獻(xiàn)介紹;傳代方法：１：３—１：６傳代，３—４天換液１次;生長特性：懸浮生長 ;形態(tài)特性：淋巴母細(xì)胞樣;相關(guān)產(chǎn)品有：MLMA細(xì)胞、H719細(xì)胞、A549/DDP細(xì)胞

OACP4C Cells;背景說明：詳見相關(guān)文獻(xiàn)介紹;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁或懸浮，詳見產(chǎn)品說明書部分;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：B-cell Acute Lymphoblastic Leukemia-1細(xì)胞、EOC 20細(xì)胞、NB-4細(xì)胞

Kit225/K6 Cells;背景說明：詳見相關(guān)文獻(xiàn)介紹;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁或懸浮，詳見產(chǎn)品說明書部分;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：J111細(xì)胞、GM00215細(xì)胞、OCI-AML4細(xì)胞

MiaPaCa-2 Cells;背景說明：詳見相關(guān)文獻(xiàn)介紹;傳代方法：１：２傳代；;生長特性：貼壁生長;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：TW-039細(xì)胞、FET細(xì)胞、Potorous tridactylus Kidney 1細(xì)胞

┈訂┈購(技術(shù)服務(wù))┈熱┈線：1┈3┈6┈4┈1┈9┈3┈0┈7┈9┈1【微信同號(hào)】┈Q┈Q：3┈1┈8┈0┈8┈0┈7┈3┈2┈4；

形態(tài)特性：上皮細(xì)胞樣

細(xì)胞凍存復(fù)蘇材料與方法步驟：常用的細(xì)胞冷凍貯存器為貯存器，規(guī)格有３５L和５０L兩種。使用時(shí)要注意以下幾點(diǎn)：(１)一般兩周需充一次，至少一個(gè)月充一次。溫度達(dá)-１９６℃，使用時(shí)注意勿讓濺到皮膚上，以免引起凍傷。(２)容器為雙層結(jié)構(gòu)，中間為真空層，瓶口有雙層焊接處，應(yīng)防止焊接部裂開。(３)在裝入時(shí)，要注意緩慢小心，并用厚紙卷筒或制漏斗作引導(dǎo)，使直達(dá)瓶底，如有專用灌注裝置則更HAO。若為初次使用，加時(shí)更要緩慢，以免溫度驟降而使容器損壞。細(xì)胞凍存時(shí)常備的材料有：０.２５%胰蛋白酶，含１０%～２０%的血清培養(yǎng)，DMSO(分析純)或無色新鮮甘油(１２１°C蒸氣GAO壓消毒)，２mL安瓿(或?qū)Ｓ眉?xì)胞凍存管)、吸管、離心管、噴燈、紗布袋(或凍存管架)等。主要操作步驟為：(１)選擇處于對(duì)數(shù)生長期的細(xì)胞，在凍存前一天ZuiHAO換。將多個(gè)培養(yǎng)瓶中的細(xì)胞培養(yǎng) 去掉，用０.２５%胰蛋白酶消化。適時(shí)去掉胰蛋白酶，加入少量新培養(yǎng)。用吸管吸取培養(yǎng)反復(fù)吹打瓶壁上的細(xì)胞，使其成為均勻分散的細(xì)胞懸。懸浮生產(chǎn)細(xì)胞則不要消化處理。然后將細(xì)胞收集于離心管中離心(１０００r/min，１０分鐘)。(２)去上清，加入含２０%小牛血清的完全培養(yǎng)基，于４℃預(yù)冷１５分鐘后，逐滴加入已無菌的DMSO或甘油，用吸管輕輕吹打使細(xì)胞均勻，細(xì)胞濃度為３×１０６～１×１０７/mL之間。(３)將上述細(xì)胞分裝于安瓿或?qū)Ｓ美鋬鏊芰瞎苤?，安瓿裝１～１.５mL在火焰噴燈上封口，封口處要完全封閉，圓滑無勾。冷凍管要將蓋子蓋緊，并標(biāo)記HAO細(xì)胞名稱和凍存日期，同時(shí)作HAO登記(日期、細(xì)胞種類及代次、凍存支數(shù))。(４)將裝HAO細(xì)胞的安瓿或凍存管裝入沙布袋內(nèi);置于容器頸口處存放過夜，次日轉(zhuǎn)入中。采用控制降溫速度的方法也可采用下列步驟：先將安瓿置入４℃冰箱中２～３小時(shí)，再移至冰箱冷凍室內(nèi)３～４小時(shí)，再吊入容器頸氣態(tài)部分存放２小時(shí)，Zui后沉入中。細(xì)胞凍存在中可以長期保存，但為妥善起見，凍存半年后，ZuiHAO取出一只安瓿細(xì)胞復(fù)蘇培養(yǎng)，觀察生長情況，然后再繼續(xù)凍存。

HEK293-EBNA Cells;背景說明：詳見相關(guān)文獻(xiàn)介紹;傳代方法：１：４-１：１０傳代；每周２次。;生長特性：貼壁生長;形態(tài)特性：上皮細(xì)胞樣;相關(guān)產(chǎn)品有：Panc 2.03細(xì)胞、Hs746T細(xì)胞、U-937細(xì)胞

WEHI 3 Cells;背景說明：白血??；BALB/c;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：懸浮;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：NCI-H292細(xì)胞、Hs27F細(xì)胞、Clone 15 HL-60細(xì)胞

H-2023 Cells;背景說明：詳見相關(guān)文獻(xiàn)介紹;傳代方法：每周換液２次;生長特性：貼壁或懸浮，詳見產(chǎn)品說明書部分;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：Kelly細(xì)胞、NCI-H102細(xì)胞、Reh細(xì)胞

TE-7 Cells;背景說明：食管鱗癌；男性;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：Human Intestinal Epithelial Cell-6細(xì)胞、MGH-UI細(xì)胞、H2291細(xì)胞

NCI-H520 Cells;背景說明：詳見相關(guān)文獻(xiàn)介紹;傳代方法：１：３-１：６傳代；２-３天換液１次;生長特性：貼壁或懸浮，詳見產(chǎn)品說明書部分;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：Hs888T細(xì)胞、DC2.4細(xì)胞、HIEC-6細(xì)胞

HMEC-1 Cells;背景說明：詳見相關(guān)文獻(xiàn)介紹;傳代方法：１：２傳代;生長特性：貼壁生長;形態(tài)特性：上皮細(xì)胞樣;相關(guān)產(chǎn)品有：LICCF細(xì)胞、5637細(xì)胞、APRE-19細(xì)胞

NUGC4 Cells;背景說明：胃癌；女性;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：半貼壁;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：HCEpiC細(xì)胞、IGROV-1細(xì)胞、TGW-nu-1細(xì)胞

HEL 299 Cells;背景說明：紅白細(xì)胞白血病;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：懸浮;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：H2085細(xì)胞、MM.1S細(xì)胞、L-5178-Y細(xì)胞

McA-RH7777 Cells;背景說明：肝癌；雌性；Buffalo;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：H-1373細(xì)胞、SUM-149細(xì)胞、SMC-1細(xì)胞

NCI-H727 Cells;背景說明：詳見相關(guān)文獻(xiàn)介紹;傳代方法：每周換液２次。;生長特性：貼壁生長;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：T-ALL 1細(xì)胞、NCI-H1993細(xì)胞、KG-1細(xì)胞

M619 Cells;背景說明：脈絡(luò)膜黑色素瘤；女性;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：H498細(xì)胞、PC-10細(xì)胞、UM-RC-2細(xì)胞

SK-MEL2 Cells;背景說明：詳見相關(guān)文獻(xiàn)介紹;傳代方法：１：３-１：６傳代，２-３天換液１次。;生長特性：貼壁生長;形態(tài)特性：多邊形的;相關(guān)產(chǎn)品有：COLO824細(xì)胞、NCIH2286細(xì)胞、NBL-2細(xì)胞

HRGEC Cells;背景說明：腎小球；內(nèi)皮 Cells;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：OCI-LY-18細(xì)胞、HTR8svn細(xì)胞、RERF-LCMS細(xì)胞

OUMS-23 Cells;背景說明：詳見相關(guān)文獻(xiàn)介紹;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁或懸浮，詳見產(chǎn)品說明書部分;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：210RCY3-Ag1.2.3細(xì)胞、SF 763細(xì)胞、COLO206F細(xì)胞

RDES-1 Cells;背景說明：詳見相關(guān)文獻(xiàn)介紹;傳代方法：１：８傳代：每周換液２-３次;生長特性：貼壁和懸浮混合;形態(tài)特性：上皮樣;相關(guān)產(chǎn)品有：HuT 78細(xì)胞、U-373-MG細(xì)胞、C-Lu-65細(xì)胞

108CC15 Cells;背景說明：詳見相關(guān)文獻(xiàn)介紹;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁或懸浮，詳見產(chǎn)品說明書部分;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：OAW 42細(xì)胞、BHP10-3細(xì)胞、RT 112細(xì)胞

Cor L51 Cells;背景說明：詳見相關(guān)文獻(xiàn)介紹;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁或懸浮，詳見產(chǎn)品說明書部分;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：NU-GC-3細(xì)胞、8305C細(xì)胞、MeSoTheliOma-211H細(xì)胞

Abcam HCT 116 GATA2 KO Cells(提供STR鑒定圖譜)

AG06846 Cells(提供STR鑒定圖譜)

BayGenomics ES cell line CSI690 Cells(提供STR鑒定圖譜)

BayGenomics ES cell line STA078 Cells(提供STR鑒定圖譜)

BIONi010-C-29 Cells(提供STR鑒定圖譜)

CIPGC Cells(提供STR鑒定圖譜)

DA03255 Cells(提供STR鑒定圖譜)

DD2173 Cells(提供STR鑒定圖譜)

GM02078 Cells(提供STR鑒定圖譜)

HSCT6 Cells;背景說明：詳見相關(guān)文獻(xiàn)介紹;傳代方法：１：２傳代;生長特性：貼壁生長;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：LSECs細(xì)胞、BI-Mel細(xì)胞、SVGp12細(xì)胞

SW620/LUC人結(jié)腸癌細(xì)胞代次低|培養(yǎng)基|送STR圖譜<熒光素酶標(biāo)記>

SU86_86 Cells;背景說明：詳見相關(guān)文獻(xiàn)介紹;傳代方法：１：２傳代；;生長特性：貼壁生長;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：HFL-I細(xì)胞、Melan-a細(xì)胞、L-Wnt3A細(xì)胞

A9 Cells;背景說明：皮下結(jié)締組織；自發(fā)永生；雄性；C３H/An;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：NCIH1688細(xì)胞、H-1573細(xì)胞、A-10細(xì)胞

RPTEC/TERT1 Cells;背景說明：腎；近端小管上皮；HGNC-TERT轉(zhuǎn)化；男性;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：PTK-2細(xì)胞、H1819細(xì)胞、NT2細(xì)胞

NFS 60 Cells;背景說明：詳見相關(guān)文獻(xiàn)介紹;傳代方法：１：３傳代;生長特性：貼壁生長;形態(tài)特性：上皮細(xì)胞樣;相關(guān)產(chǎn)品有：CHO細(xì)胞、NCI-SNU-761細(xì)胞、Malme3M細(xì)胞

3T3L1 Cells;背景說明：３T３-L１是從３T３細(xì)胞（Swissalbino）中經(jīng)克隆分離得到的連續(xù)傳代的亞系。該細(xì)胞從快速分裂到匯合和接觸性抑制狀態(tài)經(jīng)歷了前脂肪細(xì)胞到脂肪樣細(xì)胞的轉(zhuǎn)變。該細(xì)胞鼠痘病毒陰性；可產(chǎn)生甘油三酯，高濃度血清可增強(qiáng)細(xì)胞內(nèi)脂肪堆積。;傳代方法：１：２傳代;生長特性：貼壁生長;形態(tài)特性：成纖維細(xì)胞樣;相關(guān)產(chǎn)品有：CATHa細(xì)胞、RKOAS451細(xì)胞、HCC-2185細(xì)胞

42.9E5.2 Cells(提供STR鑒定圖譜)

ATDC-5 Cells;背景說明：詳見相關(guān)文獻(xiàn)介紹;傳代方法：消化３-５分鐘，１：２,３天內(nèi)可長滿;生長特性：貼壁生長;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：KMST-6細(xì)胞、OVCAR-3細(xì)胞、MBMEC細(xì)胞

P30-OHK Cells;背景說明：詳見相關(guān)文獻(xiàn)介紹;傳代方法：１０^５ cells/６０mm dish;生長特性：懸浮生長;形態(tài)特性：淋巴母細(xì)胞;相關(guān)產(chǎn)品有：MDA468細(xì)胞、KU812-F細(xì)胞、H441細(xì)胞

MDAPCa2b Cells;背景說明：詳見相關(guān)文獻(xiàn)介紹;傳代方法：１：２-１：４傳代，２-３天換液１次。;生長特性：貼壁生長;形態(tài)特性：上皮細(xì)胞;相關(guān)產(chǎn)品有：TE3細(xì)胞、JM1細(xì)胞、Lieming Xu-2細(xì)胞

Pa18C Cells;背景說明：詳見相關(guān)文獻(xiàn)介紹;傳代方法：１：２傳代;生長特性：貼壁生長;形態(tài)特性：上皮樣;相關(guān)產(chǎn)品有：HS-766T細(xì)胞、BSC40細(xì)胞、NCI-441細(xì)胞

HL1 Cells;背景說明：心肌；SV４０轉(zhuǎn)化;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：EM-3細(xì)胞、KU-812F細(xì)胞、Human Foreskin Fibroblast細(xì)胞

NCIH2135 Cells;背景說明：詳見相關(guān)文獻(xiàn)介紹;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁或懸浮，詳見產(chǎn)品說明書部分;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：MES-SA-Dx5細(xì)胞、OE-21細(xì)胞、MD Anderson-Metastatic Breast-468細(xì)胞

J82 COT Cells;背景說明：電子顯微鏡下未觀察到橋粒但觀察到數(shù)目不同的粗面內(nèi)質(zhì)網(wǎng)和突出微絲。 含ras (H-ras)癌基因。;傳代方法：１：２傳代;生長特性：貼壁生長;形態(tài)特性：上皮細(xì)胞樣;相關(guān)產(chǎn)品有：TE-1細(xì)胞、Namalva細(xì)胞、NCIH196細(xì)胞

WI-38 Cells;背景說明：LeonardHayflick建系；有限傳代細(xì)胞系；壽命為５０±１０代（倍增時(shí)間２４h）；來自妊娠３個(gè)月的正常胚胎肺組織。該細(xì)胞系是第一個(gè)用于人制備的人二倍體細(xì)胞；培養(yǎng)基中添加TNFα可以加快細(xì)胞生長。;傳代方法：１：２-１：４傳代；２-３天換液１次;生長特性：貼壁生長;形態(tài)特性：成纖維細(xì)胞樣;相關(guān)產(chǎn)品有：RD細(xì)胞、LMTK-細(xì)胞、OCI-AML3細(xì)胞

GM17731 Cells(提供STR鑒定圖譜)

HAP1 HSPB1 (-) 3 Cells(提供STR鑒定圖譜)

RPMI8402 Cells;背景說明：急性T淋巴細(xì)胞白血?。慌?傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：懸浮;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：C-33 A細(xì)胞、MDCC MSB1細(xì)胞、Rat 1細(xì)胞

Psi2 DAP Cells;背景說明：詳見相關(guān)文獻(xiàn)介紹;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁或懸浮，詳見產(chǎn)品說明書部分;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：NCIH128細(xì)胞、A9(Hamprecht)細(xì)胞、ML2細(xì)胞

SUD6 Cells;背景說明：詳見相關(guān)文獻(xiàn)介紹;傳代方法：１：３—１：６傳代，３—４天換液１次;生長特性：懸浮生長 ;形態(tài)特性：淋巴母細(xì)胞樣;相關(guān)產(chǎn)品有：NCI-H1623細(xì)胞、RCS細(xì)胞、Walker-256-TC細(xì)胞

HTh-74 Cells;背景說明：未分化甲狀腺癌；女性;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：Mel-MeWo細(xì)胞、HSC-I細(xì)胞、YD-15細(xì)胞

MPP-89 Cells;背景說明：詳見相關(guān)文獻(xiàn)介紹;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁或懸浮，詳見產(chǎn)品說明書部分;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：Adeno-293細(xì)胞、COLO 738細(xì)胞、IBRS2細(xì)胞

TE-13 Cells;背景說明：食管鱗癌；男性;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：Jurkat clone A3細(xì)胞、HOS (TE85)細(xì)胞、NRK細(xì)胞

mousepodocyte Cells;背景說明：詳見相關(guān)文獻(xiàn)介紹;傳代方法：１：２傳代;生長特性：懸浮生長;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：HH [Human lymphoma]細(xì)胞、Menschliche Und Tierische Zellkulture-3細(xì)胞、MCA-38細(xì)胞

T/G HA VSMC Cells;背景說明：詳見相關(guān)文獻(xiàn)介紹;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁或懸浮，詳見產(chǎn)品說明書部分;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：10T1/2細(xì)胞、AsPC-1細(xì)胞、HARAB細(xì)胞

HPS0172 Cells(提供STR鑒定圖譜)

JHU173i Cells(提供STR鑒定圖譜)

MDA-MB-231 25PACR Cells(提供STR鑒定圖譜)

ND34116 Cells(提供STR鑒定圖譜)

PR01241 Cells(提供STR鑒定圖譜)

Ubigene HEK293 HCK KO Cells(提供STR鑒定圖譜)

WG1534 Cells(提供STR鑒定圖譜)

HBEC1-KT Cells(提供STR鑒定圖譜)

Detroit-562 Cells;背景說明：器官：咽頭 疾?。喊?取材轉(zhuǎn)移灶：胸水;傳代方法：１：２-１：４傳代，２-３天換液１次。;生長特性：貼壁生長;形態(tài)特性：上皮細(xì)胞;相關(guān)產(chǎn)品有：T98細(xì)胞、H-35 Reuber細(xì)胞、QGP-1細(xì)胞

NCIH2126 Cells;背景說明：詳見相關(guān)文獻(xiàn)介紹;傳代方法：１：２-１：４傳代，每周２-３次。;生長特性：貼壁生長;形態(tài)特性：上皮細(xì)胞樣;相關(guān)產(chǎn)品有：GM05862細(xì)胞、H1954細(xì)胞、HS-766T細(xì)胞

H-157 Cells;背景說明：詳見相關(guān)文獻(xiàn)介紹;傳代方法：１：２傳代;生長特性：貼壁生長　;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：U266 B1細(xì)胞、NCI-H1876細(xì)胞、Panc10.05細(xì)胞

SF-539 BT Cells;背景說明：膠質(zhì)瘤；女性;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：SCL1細(xì)胞、Liver-02細(xì)胞、RMC細(xì)胞

THLE-3 Cells;背景說明：詳見相關(guān)文獻(xiàn)介紹;傳代方法：消化３-５分鐘。１：２。３天內(nèi)可長滿;生長特性：貼壁生長;形態(tài)特性：上皮樣;相關(guān)產(chǎn)品有：Human podocyte細(xì)胞、SKMES細(xì)胞、95D細(xì)胞

THLE-3 Cells;背景說明：詳見相關(guān)文獻(xiàn)介紹;傳代方法：消化３-５分鐘。１：２。３天內(nèi)可長滿;生長特性：貼壁生長;形態(tài)特性：上皮樣;相關(guān)產(chǎn)品有：Human podocyte細(xì)胞、SKMES細(xì)胞、95D細(xì)胞

GM03671C Cells;背景說明：G.E. Foley 等人建立了類淋巴母細(xì)胞細(xì)胞株CCRF-CEM。 細(xì)胞是１９６４年１１月從一位四歲白人女性急性淋巴細(xì)胞白血病患者的外周血白血球衣中得到。此細(xì)胞系從香港收集而來。;傳代方法：１：２傳代。３天內(nèi)可長滿。;生長特性：懸浮生長;形態(tài)特性：淋巴母細(xì)胞樣;相關(guān)產(chǎn)品有：JTC-28細(xì)胞、MCF7ADR細(xì)胞、U-118 MG細(xì)胞

THLE-3 Cells;背景說明：詳見相關(guān)文獻(xiàn)介紹;傳代方法：消化３-５分鐘。１：２。３天內(nèi)可長滿;生長特性：貼壁生長;形態(tài)特性：上皮樣;相關(guān)產(chǎn)品有：Human podocyte細(xì)胞、SKMES細(xì)胞、95D細(xì)胞

NCI-H2195 Cells;背景說明：詳見相關(guān)文獻(xiàn)介紹;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁或懸浮，詳見產(chǎn)品說明書部分;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：Menschliche Und Tierische Zellkulture-3細(xì)胞、HEK 293 EBNA細(xì)胞、VMCUB1細(xì)胞

H-647 Cells;背景說明：詳見相關(guān)文獻(xiàn)介紹;傳代方法：１：３-１：６傳代；每周換液２次。;生長特性：貼壁生長;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：Kit225/K6細(xì)胞、P3X63 AG 8.653細(xì)胞、NRK49F細(xì)胞

THLE-3 Cells;背景說明：詳見相關(guān)文獻(xiàn)介紹;傳代方法：消化３-５分鐘。１：２。３天內(nèi)可長滿;生長特性：貼壁生長;形態(tài)特性：上皮樣;相關(guān)產(chǎn)品有：Human podocyte細(xì)胞、SKMES細(xì)胞、95D細(xì)胞

THLE-2 Cells;背景說明：詳見相關(guān)文獻(xiàn)介紹;傳代方法：１：３-１：６傳代；２-３天換液１次。;生長特性：貼壁生長;形態(tài)特性：上皮樣;相關(guān)產(chǎn)品有：TALL-1 [Human adult T-ALL]細(xì)胞、EBC-1細(xì)胞、NPC-TW039細(xì)胞

HRMC Cells;背景說明：腎；系膜 Cells;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：Institute for Medical Research-90細(xì)胞、3T3-Swiss細(xì)胞、Hs852T細(xì)胞

BEL-7402 Cells;背景說明：BEL-７４０２細(xì)胞株是１９７４年從臨床肝癌手術(shù)標(biāo)本中建立的。;傳代方法：消化３-５分鐘，１：２,３天內(nèi)可長滿;生長特性：貼壁生長;形態(tài)特性：上皮樣;相關(guān)產(chǎn)品有：5-8F細(xì)胞、P-36細(xì)胞、Epithelioma Papulosum Cyprini細(xì)胞

OCUM-1 Cells;背景說明：詳見相關(guān)文獻(xiàn)介紹;傳代方法：１：２傳代;生長特性：懸浮生長;形態(tài)特性：淋巴母細(xì)胞;相關(guān)產(chǎn)品有：MDA-MB-157細(xì)胞、NCI-H1666細(xì)胞、HEK (AD293)細(xì)胞

SKOV-3/Luc Cells(提供STR鑒定圖譜)

NPA 87 Cells;背景說明：詳見相關(guān)文獻(xiàn)介紹;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁或懸浮，詳見產(chǎn)品說明書部分;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：L-6TG細(xì)胞、32Dcl3細(xì)胞、Swiss 3T3細(xì)胞

NCI-H209 Cells;背景說明：詳見相關(guān)文獻(xiàn)介紹;傳代方法：１：２-１：４傳代；每周換液２次。;生長特性：懸浮生長，有少數(shù)細(xì)胞疏松貼壁;形態(tài)特性：上皮樣;相關(guān)產(chǎn)品有：RA.1細(xì)胞、H1693細(xì)胞、KBM-7細(xì)胞

HCE Cells;背景說明：角膜上皮細(xì)胞；Ad-SV４０轉(zhuǎn)化；女性;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：HEC-1-A細(xì)胞、SMA560細(xì)胞、LUDLU-1細(xì)胞

H358 Cells;背景說明：１９８１年從一位開始化療之前的患者的腫瘤組織中分離建株。超微結(jié)構(gòu)研究表明細(xì)胞質(zhì)中有Clara細(xì)胞的特征結(jié)構(gòu)細(xì)胞表達(dá)主要的肺表面結(jié)合蛋白SP-A的蛋白和RNA。不表達(dá)SP-B和SP-C。他們?cè)谲洯傊械目寺⌒纬尚蕿椋?８３%。;傳代方法：消化３-５分鐘。１：２。３天內(nèi)可長滿。;生長特性：貼壁生長;形態(tài)特性：上皮樣;相關(guān)產(chǎn)品有：P3/NS1/1-Ag4.1細(xì)胞、NK-92 transfected with MFG-hIL2細(xì)胞、ACC-2細(xì)胞

GM0637 Cells;背景說明：詳見相關(guān)文獻(xiàn)介紹;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁或懸浮，詳見產(chǎn)品說明書部分;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：SUM149細(xì)胞、TOV112D細(xì)胞、Leukemic 1210細(xì)胞

B16BL-6 Cells;背景說明：黑色素瘤；雄性；C５７BL/６;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：MMQ細(xì)胞、Ca 9-22細(xì)胞、H1755細(xì)胞

SW620/LUC人結(jié)腸癌細(xì)胞代次低|培養(yǎng)基|送STR圖譜<熒光素酶標(biāo)記>

CORL88 Cells;背景說明：詳見相關(guān)文獻(xiàn)介紹;傳代方法：１：２傳代;生長特性：貼壁生長;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：H-1819細(xì)胞、Vx2細(xì)胞、MESSA/Dx5細(xì)胞

BT-549 Cells;背景說明：該細(xì)胞１９７８年由W.G.Coutinho和E.Y.Lasfargues建系，源自一位７２歲患有乳腺導(dǎo)管癌的白人女性，來源組織包括乳頭及浸潤導(dǎo)管。該細(xì)胞形態(tài)包括上皮樣細(xì)胞及多核巨細(xì)胞，可分泌一種粘性物質(zhì)。;傳代方法：１：２傳代;生長特性：貼壁生長;形態(tài)特性：上皮樣;相關(guān)產(chǎn)品有：H719細(xì)胞、Karpas-422細(xì)胞、Dami細(xì)胞

SW 1353 Cells;背景說明：骨肉瘤；女性;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：Hs 729.T細(xì)胞、WM266-4細(xì)胞、alphaTC clone 6細(xì)胞

MFD-1 Cells;背景說明：詳見相關(guān)文獻(xiàn)介紹;傳代方法：維持細(xì)胞濃度在３×１０４-５×１０５ cells/ml；２-３天換液１次。;生長特性：懸浮生長;形態(tài)特性：淋巴母細(xì)胞樣　;相關(guān)產(chǎn)品有：LAPC-4細(xì)胞、H-128細(xì)胞、HCC0038細(xì)胞

HUT-125 Cells;背景說明：腺鱗狀肺癌；男性;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：HEL-92_1_7細(xì)胞、HCC0366細(xì)胞、NCI-H841細(xì)胞

HS 683T Cells;背景說明：該細(xì)胞源自７６歲白人男性的左顳葉側(cè)膠質(zhì)瘤組織，有微絨毛，無橋粒。 ;傳代方法：１：４傳代，每周換液２次;生長特性：貼壁生長;形態(tài)特性：成纖維細(xì)胞;相關(guān)產(chǎn)品有：NCI-H522細(xì)胞、MCF-12F細(xì)胞、SKRC-52細(xì)胞

HCC-1395 Cells;背景說明：詳見相關(guān)文獻(xiàn)介紹;傳代方法：１：２傳代，每周換液２—３次;生長特性：貼壁生長;形態(tài)特性：上皮樣;相關(guān)產(chǎn)品有：Calu3細(xì)胞、CTLL(2)細(xì)胞、MHCC97H細(xì)胞

HIT T15 Cells;背景說明：胰島β細(xì)胞；SV４０轉(zhuǎn)化;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：GP2d細(xì)胞、NCIH2347細(xì)胞、HCC-1438細(xì)胞

BayGenomics ES cell line RRF280 Cells(提供STR鑒定圖譜)

BayGenomics ES cell line XG499 Cells(提供STR鑒定圖譜)

EC-Trpm5tm1Dgen Cells(提供STR鑒定圖譜)

MQ49 Cells(提供STR鑒定圖譜)

TPC417 Cells(提供STR鑒定圖譜)

M4Be T9P76 Cells(提供STR鑒定圖譜)

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