Sequencing Grade Carboxypeptidase B
Catalog Number:SRCPB01
CAS:9025-24-5
EC:3.4.17.2
Storage temperature: 2-8℃
Source: Sequencing grade recombinant carboxypeptidase B is a genetically engineered protein expressed in E.coli
Protein Sequence:The amino acid sequence of recombinant carboxypeptidase B is identical to rat carboxypeptidase B
1.DESCRIPTION
Carboxypeptidase B catalyzes hydrolysis of the basic amino acids lysine, arginine and histidine from the C-terminal end of polypeptides. The molecular weight is 33.8kD, pI is 6.0 and the optimum pH is 7.5-9.0. Carboxypeptidase B is competitively inhibited by arginine and lysine. The enzyme is also inhibited by metal chelating agents, e.g., EDTA.
2. MAIN FEATURES
3.RECOMMEND USAGE
Prepare 1-10mg/ml carboxypeptidase B with sterile water or 25mM Tris-HCl pH 7.65. The ratio to aimed protein is 1:50 to 1:1000 (w/w), the optimum pH is pH 7.5-9.0.
4.STABILITY OF STORAGE AND TRANSPORT
Stability of storage: Sequencing grade carboxypeptidase B lyophilized should be stored under 2-8℃ in sealed container. It is stable within 24 months.After dissolved with sterile water or 25mM Tris-HCl, it should be stored under -20℃. Avoid repeated freezing and thawing.
Stability of transport: The product is stable by blue ice insulation transport.
5.PRODUCT ADVANTAGE
- Sequence grade recombinant protein: high specificity,good stability.
- Animal origin free: Sequencing grade carboxypeptidase B is no exogenous virus contamination,and any animal origin material is not used in the production process.
- Stable quality: Mass production can ensure stable and continuous batch production.It is no difference between the batch and the product quality is stable.
- High purity: Higher specific activity.Host protein residues is less than the limits of biological products.
- Lyophilized powder: The product is lyophilized powder and is easy to store and transport.
- Compliance with regulatory requirements: Production equipment and production environment comply with relevant regulatory requirements, and the production process is in full compliance with NSF ISO 9001: 2008 quality system and GMP guidelines.
- Complete quality documents: we can provide relevant regular support files in according to customers’ requirement.
6.PRODUCT USAGE
1) Determination of protein C-terminal basic amino acids such as arginine, lysine and histidine.
2) detection of antibody basic peak.
7.RELATED PRODUCTS
Recombinant trypsin
Sequencing grade trypsin
Recombinant human trypsin
Recombinant enterokinase
關(guān)鍵字： Carboxypeptidase B；Sequencing Grade carboxypeptidase B
上海雅心生物技術(shù)有限公司主要從事基因工程蛋白酶的研發(fā)和生產(chǎn)，目前的主要品種為基因工程羧肽酶B、基因工程胰蛋白酶和基因工程脂肪酶A；為本公司自主研發(fā)，擁有自主知識產(chǎn)權(quán)。公司擁有自組研發(fā)團(tuán)隊(duì)，擁有世界先進(jìn)的重組生物技術(shù)；自主研發(fā)的重組生物用酶為世界上生物用酶的首創(chuàng)！
基因工程羧肽酶B，基因工程胰蛋白酶和基因工程脂肪酶A都是由基因工程方法生產(chǎn)，純度高，不含雜酶，無羧肽酶A、糜蛋白酶等其他蛋白酶活性；無動物源性，出口產(chǎn)品無需進(jìn)行終產(chǎn)品的動物源性檢驗(yàn)。制品中不含蛋白酶抑制劑，產(chǎn)品安全。
公司秉承“科技致勝”的理念，堅持“提供高純度重組蛋白酶”，并愿與廣大同行交流，有需求的客戶提供優(yōu)質(zhì)的服務(wù)！
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