Human PSME3 / PA28g Protein, His Tag

分子別名（Synonym）

PSME3,PA28gamma,REG-gamma

表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

Human PSME3, His Tag (PS3-H5142) is expressed from E. coli cells. It contains AA Ala 2 - Tyr 254 (Accession # P61289-1).

Predicted N-terminus: Met

蛋白結(jié)構(gòu)（Molecular Characterization）

This protein carries a polyhistidine tag at the N-terminus.

The protein has a calculated MW of 30.3 kDa. The protein migrates as 28-33 kDa under reducing (R) condition (SDS-PAGE).

內(nèi)毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

純度（Purity）

>95% as determined by SDS-PAGE.

制劑（Formulation）

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重構(gòu)方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲(chǔ)（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

蛋白酶體激活物復(fù)合物亞基3（PSME3）是PA28家族的成員，也稱為11S調(diào)節(jié)物復(fù)合物γ亞基（REGγ）、多催化蛋白酶亞基3的激活劑和蛋白酶體激活劑28亞基γ（PA28γ）。PSME3激活蛋白酶體的胰蛋白酶樣催化亞基，但抑制胰凝乳蛋白酶樣和后谷氨酰偏好（PGPH）亞基。PSME3還可以促進(jìn)MDM2-p53/TP53相互作用，促進(jìn)p53/TP53的泛素化和MDM2依賴性蛋白酶體降解，限制其積累，導(dǎo)致DNA損傷后細(xì)胞凋亡受到抑制。此外，PSME3也可能參與細(xì)胞周期調(diào)控。