Mouse AGR2 / AG-2 Protein, His Tag (MALS verified)

分子別名（Synonym）

AGR2,AG-2

表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

Mouse AGR2, His Tag (AG2-M52H6) is expressed from human 293 cells (HEK293). It contains AA Lys 21 - Leu 175 (Accession # O88312-1).

Predicted N-terminus: Lys 21

蛋白結(jié)構(gòu)（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 19.8 kDa. The protein migrates as 23 kDa and 24 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

內(nèi)毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

純度（Purity）

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制劑（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重構(gòu)方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲(chǔ)（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

前梯度蛋白2（AGR2）是一種正常的內(nèi)質(zhì)網(wǎng)蛋白，具有兩棲動(dòng)物肢體再生和促進(jìn)癌癥轉(zhuǎn)移兩種重要的異常功能。這些正常的細(xì)胞內(nèi)和異常的細(xì)胞外作用可歸因于AGR2的多結(jié)構(gòu)域結(jié)構(gòu)。NMR結(jié)構(gòu)顯示AGR2由一個(gè)非結(jié)構(gòu)化的N端區(qū)域和一個(gè)硫氧還蛋白折疊組成。