HTT產(chǎn)品信息

英文名稱：Huntingtin

中文名稱：亨廷頓蛋白

靶點(diǎn)別稱：HTT

物種：Human

標(biāo)簽：His Tag & MBP Tag

屬性：Protein

標(biāo)記：Unconjugated

表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

Human HTT, MBP,His Tag (HTT-H51M5) is expressed from E. coli cells. It contains AA Met 1 - Glu 82 (Accession # P42858-1).

Predicted N-terminus: Met

蛋白結(jié)構(gòu)（Molecular Characterization）

This protein carries a MBP tag at the N-terminus and a polyhistidine tag at the C-terminus

The protein has a calculated MW of 52.7 kDa. The protein migrates as 60-63 kDa under reducing (R) condition (SDS-PAGE).

內(nèi)毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

純度（Purity）

>95% as determined by SDS-PAGE.

制劑（Formulation）

Lyophilized from 0.22 μm filtered solution in 20 mM HEPES, 150 mM NaCl, pH7.5 with trehalose as protectant.

Contact us for customized product form or formulation.

重構(gòu)方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲(chǔ)（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

亨廷頓蛋白是由HTT基因編碼的蛋白質(zhì)。亨廷頓蛋白（Htt）中的常染色體顯性突變是亨廷頓氏病（HD）的病因。在HD中，蛋白質(zhì)N端序列中的聚谷氨酰胺（polyQ）結(jié)構(gòu)域擴(kuò)展到超過36個(gè)谷氨酰胺的閾值。突變型polyQ擴(kuò)增與發(fā)病年齡呈負(fù)相關(guān)。