補(bǔ)骨脂二氫黃酮|T3846|TargetMol

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價(jià)格	￥143	￥188	￥289
包裝	5mg	10mg	25mg

最小起訂量	1mg
發(fā)貨地	上海
更新日期	2025-03-12

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產(chǎn)品詳情

中文名稱:補(bǔ)骨脂二氫黃酮	英文名稱:Bavachin
CAS:19879-32-4	品牌: TargetMol
產(chǎn)地: 美國(guó)	保存條件: Powder: -20°C for 3 years \| In solvent: -80°C for 1 year \| Shipping with blue ice.
純度規(guī)格: 100%	產(chǎn)品類別: 抑制劑
貨號(hào): T3846

Product Introduction

Bioactivity

名稱	Bavachin
描述	Bavachin (Corylifolin) is a phytoestrogen that activates the estrogen receptors ERα and ERβ.
細(xì)胞實(shí)驗(yàn)	Bavachin is dissolved in DMSO. MTT solution (20 μL) is added to each well of the 96-well plates, the cells are cultured for 4 h, the solution is discarded, and the purple crystal is dissolved in the wells with 150 μL DMSO solution, agitated in a 37°C incubator shaker for 10 min, and the optical density (OD) is measured at 490 nm by the microplate reader.
激酶實(shí)驗(yàn)	The chemiluminescent assay is used to confirm PCSEE MAO-A and MAO-B inhibitory effects and to test BNN and BVN hMAO-A and hMAO-B inhibition using MAO-Glo kit. Each enzyme's Arbitrary Light Unit (ALU) is measured in the presence of PCSEE, BNN, BVN, and standard DEP as an MAO-BI positive control. Briefly, hMAO-A and hMAO-B isozymes are diluted to 2× with reaction buffer (pH 7.4) and preincubated with 4× PCSEE, BNN, BVN, or DEP working solutions at RT for 30?min in white opaque 96-well plates. For determining activity inhibition, final 8.5?μg/mL concentrations of PCSEE, BNN, BVN, and DEP are used. For IC50 determination, 8× PCSEE and BNN working solutions are serially diluted using reaction buffers (pH 7.4) to make a 4× concentration. Ten points' range of PCSEE (1.0 to 250.0?μg/mL) and BNN (up to 400?μM (135.4?μg/mL)) final concentrations is used. Controls used are with and without ethanol. Ethanol solvent in controls is kept to a maximum final (volume) of ≤2%. Each isozyme is substituted with the reaction buffer for the blank. Based on our preliminary optimizations and Valley's method, the reaction is initiated by adding 4× luciferin derivative substrate (LDS) for a final (concentration) of 40 and 4?μM for hMAO-A and hMAO-B reactions, respectively. The final volume per well of each reaction is 50?μL. The reaction is optimized for the amount of A and B enzyme used to be incubated for less than 3.5?h at RT. To stop the reaction and produce the luminescence signal RLDR is added to all wells, 50?μL to each well, and incubated for a further 30?min.
體外活性	Bavachin顯著抑制黑色素合成和TYR活性。在10 μM濃度下，Bavachin抑制A375細(xì)胞中TYR和JNK蛋白的表達(dá)，以及TYR, TRP-1, TRP-2, ERK1, ERK2和JNK2 mRNA的表達(dá)。ICI182780和U0126能顯著逆轉(zhuǎn)Bavachin對(duì)這些蛋白和mRNA表達(dá)水平的影響[1]。在ORO染色實(shí)驗(yàn)中，Bavachin以劑量依賴的方式積累脂質(zhì)。與對(duì)照細(xì)胞相比，10 μM Bavachin顯著增加前脂肪細(xì)胞的生長(zhǎng)，這一結(jié)果通過(guò)MTT實(shí)驗(yàn)確認(rèn)。Bavachin還增加了前脂肪細(xì)胞增殖過(guò)程中新合成DNA中BrdU的摻入，胰島素可以增強(qiáng)BrdU的摻入，而2和10 μM Bavachin與胰島素共處理效果更佳。Bavachin激活脂肪生成因子并增加分化脂肪細(xì)胞中PPARγ的轉(zhuǎn)錄活性。通過(guò)Akt和AMPK途徑，Bavachin增強(qiáng)胰島素刺激的GLUT4介導(dǎo)的葡萄糖攝取[2]。BVN顯著增加hMAO-A和hMAO-B活性[3]。Bavachin在與重組ER的[3H] E2競(jìng)爭(zhēng)位點(diǎn)結(jié)合實(shí)驗(yàn)中表現(xiàn)出ER配體結(jié)合活性。通過(guò)使用ERα或ERβ和雌激素反應(yīng)性熒光素酶質(zhì)粒在CV-1細(xì)胞中的瞬時(shí)轉(zhuǎn)染體系，測(cè)定了Bavachin的雌激素活性，分別為EC50 320 nM和680 nM。Bavachin增加雌激素反應(yīng)性基因如pS2和PR的mRNA水平，并通過(guò)蛋白酶體途徑降低ERα的蛋白水平[4]。
存儲(chǔ)條件	Powder: -20°C for 3 years \| In solvent: -80°C for 1 year \| Shipping with blue ice.
溶解度	DMSO : 58 mg/mL (178.8 mM) Chloroform, Dichloromethane, Ethyl Acetate : Soluble
關(guān)鍵字	Bavachin \| Estrogen Receptor/ERR \| Inhibitor \| inhibit
相關(guān)產(chǎn)品	Astragaloside IV \| Natamycin \| Estradiol \| Melatonin \| Mifepristone \| Estradiol benzoate \| Ethisterone \| Tamoxifen \| Allura Red AC \| Kaempferol \| Cholesterol \| Chrysin
相關(guān)庫(kù)	經(jīng)典已知活性庫(kù) \| 植物來(lái)源化合物庫(kù) \| 已知活性化合物庫(kù) \| 黃酮類天然產(chǎn)物庫(kù) \| 抗癌天然產(chǎn)物庫(kù) \| 天然產(chǎn)物庫(kù) \| 中藥單體化合物庫(kù) \| 核受體化合物庫(kù) \| 高通量篩選天然產(chǎn)物庫(kù)

關(guān)鍵字：補(bǔ)骨脂甲素|||補(bǔ)骨脂二氫黃酮|||Corylifolin|TargetMol

公司簡(jiǎn)介

上海陶術(shù)生物科技有限公司為美國(guó)Target Molecule Corp. ( Target Mol ) 在上海建立的全資子公司。我們與美國(guó)波士頓、德國(guó)慕尼黑的同事一起，為北美、歐洲和亞洲從事藥物研發(fā)和生物學(xué)研究的科學(xué)家提供優(yōu)質(zhì)的產(chǎn)品和專業(yè)的服務(wù)。公司下設(shè)篩選事業(yè)部，化學(xué)事業(yè)部，生物事業(yè)部和新材料部。從虛擬篩選到實(shí)體化合物分子供應(yīng)；從商業(yè)化產(chǎn)品銷售到個(gè)性化定制合成；從對(duì)明確靶點(diǎn)的分子篩選到對(duì)明確分子的多靶點(diǎn)篩選，從高通量篩選到化學(xué)結(jié)構(gòu)優(yōu)化，我們都可以滿足您的科研用品及技術(shù)服務(wù)的需求。經(jīng)過(guò)在中國(guó)市場(chǎng)五年的精心耕耘，我們已成為篩選化合物領(lǐng)域優(yōu)秀的供應(yīng)商，為超過(guò)五百家學(xué)校和各類企業(yè)提供了品質(zhì)卓越的小分子化合物和藥物篩

成立日期	2013-04-18 （12年）	注冊(cè)資本	566.2651萬(wàn)人民幣
員工人數(shù)	100-500人	年?duì)I業(yè)額	￥ 1億以上
主營(yíng)行業(yè)	化學(xué)試劑,生物活性小分子	經(jīng)營(yíng)模式	貿(mào)易,試劑,定制,服務(wù)

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公司成立：12年
注冊(cè)資本：566.2651萬(wàn)人民幣
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公司地址：上海市閘北區(qū)江場(chǎng)三路28號(hào)4樓

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