氯硝柳胺|T0711|TargetMol

價(jià)格	￥320
包裝	500mg

最小起訂量	1mg
發(fā)貨地	上海
更新日期	2024-12-02

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產(chǎn)品詳情

中文名稱:氯硝柳胺	英文名稱:Niclosamide
CAS:50-65-7	品牌: TargetMol
產(chǎn)地: 美國(guó)	保存條件: keep away from moisture \| Powder: -20°C for 3 years \| In solvent: -80°C for 1 year \| Shipping with blue ice.
純度規(guī)格: 96.83%	產(chǎn)品類別: 抑制劑
貨號(hào): T0711

Product Introduction

Bioactivity

名稱	Niclosamide
描述	Niclosamide (Niclocide)(BAY2353) is used to treat most tapeworm infections by inhibiting DNA replication.
細(xì)胞實(shí)驗(yàn)	Cells are plated in 96-well culture plates with cell density of 3-4 × 103 cells/well and treat with Niclosamide by adding 100 μL medium containing Niclosamide of various concentrations on the second day. After 72-hour's treatment, MTT is added to each well and incubated for additional 4-5 hours, and the absorbance is measured on a microplate reader at 570 nM. Cell growth inhibition is evaluated as the ratio of the absorbance of the sample to that of the control. The results are representative of at least 3 independent experiments. (Only for Reference)
激酶實(shí)驗(yàn)	Protein Kinase profiling assay: Assay for 22 different proteins kinases is carried out by ProQinase Gmbh. All of the protein kinases are expressed either in Sf9 insect cells or in E.coli as recombinant GST-fusion proteins or His-tagged proteins. Protein kinases are purified by affinity chromatography using either GSH-agarose or Ni_NTH-agarose. A radiometric protein kinase assay is used for measuring the kinase activity of the 22 protein kinases. Briefly, for each protein kinase, 50 μL reaction cocktail containing 60 mM HEPES-NaOH, 3 mM MgCl2, 3 mM MnCl2, 3 μM Na-orthovanadate, 1.2 mM DTT, 50 μg/mL PEG20000, 1 μM [γ-33P]-ATP, Niclosamide, adequate amount of enzyme and its substrate. The PKC-alpha assay additionally contain 1 mM Cacl2, 4 mM EDTA, 5 μg/mL phosphatidylserine and 1 μg/mL 1, 2-Dioleyl-glycerol. The reaction cocktails are incubated at 37 °C for 60 minutes and stop with 50 μL 2% (v/v) H3PO4. Incorporation of 33Pi is determined with a microplate scintillation counter. The activities and the IC50 values are calculated using Quattro Workflow V2.28.
體外活性	Niclosamide (< 5 μM) 在HeLa細(xì)胞中劑量依賴性抑制STAT3介導(dǎo)的熒光素酶報(bào)告活性，其IC50為0.25 μM。Niclosamide (< 2 μM) 在Du145細(xì)胞中劑量依賴性抑制STAT3的磷酸化。Niclosamide (1 μM) 抑制在Du145細(xì)胞中EGF誘導(dǎo)的STAT3核內(nèi)轉(zhuǎn)移。Niclosamide (< 2 μM) 在Du145細(xì)胞中劑量依賴性抑制STAT3下游基因的轉(zhuǎn)錄。Niclosamide (< 10 μM) 在Du145癌細(xì)胞中劑量依賴性誘導(dǎo)G0/G1停滯和凋亡。[1] Niclosamide能夠在感染SARS-CoV的Vero E6細(xì)胞中以微摩爾濃度抑制SARS-CoV的復(fù)制。[2] Niclosamide (< 7.5 μM) 促進(jìn)Frizzled1的內(nèi)吞作用，下調(diào)Dishevelled-2蛋白，并在U2OS細(xì)胞中抑制Wnt3A刺激的beta-catenin穩(wěn)定性和LEF/TCF報(bào)告活性。[3] Niclosamide在U2OS細(xì)胞中劑量和時(shí)間依賴性抑制TNF誘導(dǎo)的NF-κB報(bào)告活性。Niclosamide (125 nM) 抑制由p65、IKKα、IKKβ、IKKγ和TAK1在U2OS細(xì)胞中誘發(fā)的NF-κB激活。Niclosamide (< 500 nM) 完全阻斷在HL-60細(xì)胞中TNFα誘導(dǎo)的NF-κB–DNA復(fù)合體的時(shí)間和劑量依賴性變化。Niclosamide (< 10 nM) 抑制U266細(xì)胞中固有的NF-κB激活。Niclosamide在HL-60、Molm13或AML原發(fā)細(xì)胞中劑量和時(shí)間依賴性抑制TNF誘導(dǎo)的IκBα降解和p65重新定位。Niclosamide (500 nM) 降低 TNF誘導(dǎo)的與HL-60細(xì)胞生存相關(guān)的NF-κB依賴性基因產(chǎn)品。Niclosamide劑量依賴性抑制AML細(xì)胞的生長(zhǎng)并有效誘發(fā)凋亡，降低Mcl-1和XIAP水平，增加細(xì)胞內(nèi)ROS水平。[4]
體內(nèi)活性	Niclosamide（每日每公斤40毫克，腹腔注射）抑制裸鼠體內(nèi)荷HL-60異種移植瘤的AML細(xì)胞生長(zhǎng)。[4]
存儲(chǔ)條件	keep away from moisture \| Powder: -20°C for 3 years \| In solvent: -80°C for 1 year \| Shipping with blue ice.
溶解度	10% DMSO+40% PEG300+5% Tween 80+45% Saline : 0.63 mg/mL (1.93 mM), Please add co-solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. DMSO : 6.25 mg/mL (19.11 mM)
關(guān)鍵字	SW-13 \| antihelminthic \| BAY-2353 \| inhibit \| BD140A \| Parasite \| Antibiotic \| BAY 2353 \| STAT \| NCI-H295R \| Vero E6 \| Hela \| Niclosamide \| Inhibitor \| bayluscide
相關(guān)產(chǎn)品	G-418 disulfate \| Doxycycline \| Neomycin sulfate \| Metronidazole \| Hydroxychloroquine \| Nitazoxanide \| EDTA copper(II) disodium salt \| Ampicillin sodium \| Sulfamethoxazole sodium \| Kanamycin sulfate \| Kojic acid \| Kaempferol
相關(guān)庫(kù)	抑制劑庫(kù) \| 抗癌上市藥物庫(kù) \| 抗癌活性化合物庫(kù) \| 抗病毒庫(kù) \| FDA 上市藥物庫(kù) \| 抗寄生蟲庫(kù) \| 藥物功能重定位化合物庫(kù) \| 抗癌藥物庫(kù) \| 農(nóng)藥化合物庫(kù)

關(guān)鍵字：氯硝柳胺|||Niclocide|||BAY2353|TargetMol

公司簡(jiǎn)介

上海陶術(shù)生物科技有限公司為美國(guó)Target Molecule Corp. ( Target Mol ) 在上海建立的全資子公司。我們與美國(guó)波士頓、德國(guó)慕尼黑的同事一起，為北美、歐洲和亞洲從事藥物研發(fā)和生物學(xué)研究的科學(xué)家提供優(yōu)質(zhì)的產(chǎn)品和專業(yè)的服務(wù)。公司下設(shè)篩選事業(yè)部，化學(xué)事業(yè)部，生物事業(yè)部和新材料部。從虛擬篩選到實(shí)體化合物分子供應(yīng)；從商業(yè)化產(chǎn)品銷售到個(gè)性化定制合成；從對(duì)明確靶點(diǎn)的分子篩選到對(duì)明確分子的多靶點(diǎn)篩選，從高通量篩選到化學(xué)結(jié)構(gòu)優(yōu)化，我們都可以滿足您的科研用品及技術(shù)服務(wù)的需求。經(jīng)過在中國(guó)市場(chǎng)五年的精心耕耘，我們已成為篩選化合物領(lǐng)域優(yōu)秀的供應(yīng)商，為超過五百家學(xué)校和各類企業(yè)提供了品質(zhì)卓越的小分子化合物和藥物篩

成立日期	2013-04-18 （12年）	注冊(cè)資本	566.2651萬(wàn)人民幣
員工人數(shù)	100-500人	年?duì)I業(yè)額	￥ 1億以上
主營(yíng)行業(yè)	化學(xué)試劑,生物活性小分子	經(jīng)營(yíng)模式	貿(mào)易,試劑,定制,服務(wù)

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公司成立：12年
注冊(cè)資本：566.2651萬(wàn)人民幣
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主營(yíng)產(chǎn)品：小分子抑制劑，藥物篩選化合物庫(kù)，天然產(chǎn)物，活性分子化合物等
公司地址：上海市閘北區(qū)江場(chǎng)三路28號(hào)4樓

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