Mouse Complement C5 Protein, His Tag
分子別名（Synonym）
Complement C5,C5,CPAMD4
表達區(qū)間及表達系統(tǒng)（Source）
Mouse Complement C5, His Tag (CO5-M52H4) is expressed from human 293 cells (HEK293). It contains AA Gln 19 - Glu 1680 (Accession # P06684-1).
Predicted N-terminus: Gln 19 (β chain) & Asn 679 (α chain)
蛋白結構（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus.
The mature form of Complement C5 is a disulfide-linked heterodimer composed of proteolytically cleaved α and β chain. Each α and β chain has a calculated MW of 73.5 kDa (β chain) and 114.6 kDa (α chain). The protein migrates as 66 kDa (β chain) and 100 kDa (α chain) under reducing (R) condition (SDS-PAGE) due to glycosylation.
內毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
純度（Purity）
>95% as determined by SDS-PAGE.
制劑（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重構方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存儲（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
背景（Background）
C5過敏毒素來源于補體C5的蛋白水解降解，是局部炎癥過程的介質。C5前體首先通過去除4個堿性殘基進行加工，形成由二硫鍵連接的兩條鏈，β和α。C5轉化酶通過切割α鏈激活C5，釋放C5a過敏毒素并產生C5b（β鏈+α'鏈）。C5轉化酶激活C5啟動晚期補體成分C5-C9自發(fā)組裝成膜攻擊復合物。C5b具有C6的瞬時結合位點。C5b-C6絡合物是裂解絡合物組裝的基礎。C5a過敏毒素與C5AR1和蜱補體抑制劑相互作用。C5a也是一種強效的趨化因子，可以刺激多形核白細胞的運動，并引導它們向炎癥部位遷移。
關鍵字： Complement C5;補體C5;補體C5蛋白;ACRO;百普賽斯;
百普賽斯集團ACROBiosystems Group（股票代碼：301080）是成立于2010年的跨國生物科技公司，是為全球生物醫(yī)藥、健康產業(yè)領域提供關鍵生物試劑產品及解決方案的行業(yè)平臺型基石企業(yè)。2021年在創(chuàng)業(yè)板上市。百普賽斯集團業(yè)務遍布全球，橫跨亞洲、北美洲、歐洲，在中國、美國、瑞士等12個城市設有辦公室、研發(fā)中心及生產基地。目前累計服務客戶超6000家，與全球Top 20醫(yī)藥企業(yè)均建立了長期、穩(wěn)定的合作伙伴關系。集團旗下?lián)碛衅放艫CROBiosystems百普賽斯、bioSeedin柏思薈、Condense Capital墾拓資本和ACRODiagnostics百斯醫(yī)學等。