Recombinant Protein G, His Tag
分子別名(Synonym)
RPG,Protein G
表達(dá)區(qū)間及表達(dá)系統(tǒng)(Source)
Recombinant Protein G, His Tag, also known as Recombinant Streptococcus sp. Protein G was expressed in E. coli at ACRObiosystems.
蛋白結(jié)構(gòu)(Molecular Characterization)
Recombinant Protein G, His Tag is fused with the polyhistidine tag at N-terminus and a single cysteine at C-terminus. The reducing (R) protein migrates as a 29 kDa polypeptide. Protein G Protein can bind to human IgG1 Fc.
內(nèi)毒素(Endotoxin)
Less than 1.0 EU per μg by the LAL method.
純度(Purity)
>98% as determined by SDS-PAGE.
制劑(Formulation)
Lyophilized from 0.22 μm filtered solution in 100 mM NaH2PO4, pH8.5 with trehalose as protectant.
Contact us for customized product form or formulation.
重構(gòu)方法(Reconstitution)
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存儲(Storage)
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
背景(Background)
Protein G是一種免疫球蛋白結(jié)合蛋白,在C組和G組鏈球菌中表達(dá),與A蛋白非常相似,但具有不同的特異性。它是一種65 kDa(G148蛋白G)和58 kDa(C40蛋白G)的細(xì)胞表面蛋白,通過與Fc區(qū)結(jié)合在純化抗體中得到了應(yīng)用。天然分子也結(jié)合白蛋白,然而,由于血清白蛋白是抗體來源的主要污染物,白蛋白結(jié)合位點(diǎn)已從這種重組形式的Protein G中去除。
關(guān)鍵字: Protein G;Protein G蛋白;RPG蛋白;ACRO;百普賽斯;
百普賽斯集團(tuán)ACROBiosystems Group(股票代碼:301080)是成立于2010年的跨國生物科技公司,是為全球生物醫(yī)藥、健康產(chǎn)業(yè)領(lǐng)域提供關(guān)鍵生物試劑產(chǎn)品及解決方案的行業(yè)平臺型基石企業(yè)。2021年在創(chuàng)業(yè)板上市。百普賽斯集團(tuán)業(yè)務(wù)遍布全球,橫跨亞洲、北美洲、歐洲,在中國、美國、瑞士等12個城市設(shè)有辦公室、研發(fā)中心及生產(chǎn)基地。目前累計服務(wù)客戶超6000家,與全球Top 20醫(yī)藥企業(yè)均建立了長期、穩(wěn)定的合作伙伴關(guān)系。集團(tuán)旗下?lián)碛衅放艫CROBiosystems百普賽斯、bioSeedin柏思薈、Condense Capital墾拓資本和ACRODiagnostics百斯醫(yī)學(xué)等。