Human IgE Fc Protein, His Tag

分子別名（Synonym）

IgE

表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

Human IgE Fc, His Tag (IGE-H52H9) is expressed from human 293 cells (HEK293). It contains AA Ser 106 - Gly 427 (Accession # P01854-1).

Predicted N-terminus: Ser 106

蛋白結(jié)構(gòu)（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 37.6 kDa. The protein migrates as 45-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

內(nèi)毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

純度（Purity）

>95% as determined by SDS-PAGE.

制劑（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重構(gòu)方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

作為五種指定的免疫球蛋白同種型之一，免疫球蛋白E（IgE）通過誘導(dǎo)即時超敏反應(yīng)在特應(yīng)性疾病中起著重要作用。IgE還對人體對寄生蟲感染的免疫反應(yīng)做出了重大貢獻(xiàn)。IgE抗體主要存在于組織中，通過高親和力IgE-Fc受體（Fc-epsilon RI）和低親和力IgE受體（Fc-epsilon RII）牢固地附著在效應(yīng)細(xì)胞上，如肥大細(xì)胞和嗜堿性粒細(xì)胞。