米哚妥林|T3211

價格	￥627	￥1490	￥2390
包裝	1mg	5mg	10mg

最小起訂量	1mg
發(fā)貨地	上海
更新日期	2024-12-02

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產(chǎn)品詳情

中文名稱:米哚妥林	英文名稱:Midostaurin
CAS:120685-11-2	品牌: TargetMol
產(chǎn)地: 美國	保存條件: Powder: -20°C for 3 years \| In solvent: -80°C for 1 year \| Shipping with blue ice.
純度規(guī)格: 99.56%	產(chǎn)品類別: 抑制劑
貨號: T3211

Product Introduction

Bioactivity

名稱	Midostaurin
描述	PKC412(Midostaurin (PKC412); CGP41231; CGP41251) is a broad spectrum protein kinase inhibitor. Midostaurin inhibits protein kinase C alpha (PKCalpha), vascular endothelial growth factor receptor 2 (VEGFR2), c-kit, platelet-derived growth factor receptor (PDGFR) and FMS-like tyrosine kinase 3 (FLT3) tyrosine kinases, which may result in disruption of the cell cycle, inhibition of proliferation, apoptosis, and inhibition of angiogenesis in susceptible tumors.
細(xì)胞實驗	Each well is added with 5 mM WST-1 and 0.2 mM 1-methoxy PMS and the absorbance at 450 nm is measured by a Microplate Reader.(Only for Reference)
激酶實驗	Cells are plated overnight and treated with 100 nM AZD3965 or vehicle for 24 hours. The cells are then washed, once in PBS and twice with lysis buffer (50 mM Mops, 100 mM KCl, 5 mM MgCl2, 1 mM EDTA, 0.1 mM DTT, 1 mM PMSF supplemented with 1× mini complete protease inhibitor cocktail tablets. The cells are harvested by scraping and centrifugation, and the pellet snap frozen without buffer in liquid nitrogen. Frozen aliquots of cells are thawed on ice and re-suspended in lysis buffer. Cells are lysed by 3 rounds of freezing in liquid nitrogen and thawing at 37°C for 2 minutes each. Lysates are subsequently centrifuged (13000 g, 10min, 4°C) until clear and then stored on ice. Enzyme activity in the cell lysates is determined using a micro-plate reader to measure either production or depletion of NADH/NADPH, through its absorbance at 340/10 nm, occurring as a result of direct or coupled enzyme reactions. The 96 well plates used for the assays are pre-heated to 37°C for 10 minutes prior to starting reactions, initiated by the addition of 5 μL cell lysate to 95 μL of reaction buffer (50 mM Mops pH 7.4, 100 mM KCl, 5 mM free magnesium). The standard reaction buffer is supplemented to assay the kinetics of the different enzymes. Absorbance values for controls are subtracted from absorbance of corresponding reactions. Graphpad prism 6 is used to plot V0 values against substrate concentration and determine Vmax and Km values. The Vmax is then normalised to the protein concentration in the cell lysate[1].
體外活性	Midostaurin(pkc412)是一種廣譜蛋白激酶抑制劑。它與常見的PKC-α、-β、-γ、PDGFRβ、VEGF-R2、VEGF-R1以及細(xì)胞周期依賴性激酶1-細(xì)胞周期蛋白B復(fù)合體的ATP結(jié)合位點有強烈的相互作用。Midostaurin(pkc412)能夠在亞微摩爾濃度下抑制各種人類和動物細(xì)胞系體外生長。此外，Midostaurin(pkc412)有效抑制了膠質(zhì)母細(xì)胞瘤體外增殖，并誘導(dǎo)細(xì)胞在G2/M期積累及巨核形成，伴隨廣泛的碎片化和凋亡體生成。Midostaurin(pkc412)還能逆轉(zhuǎn)腫瘤細(xì)胞體外p-糖蛋白介導(dǎo)的多藥耐藥性。[1]
體內(nèi)活性	Midostaurin(pkc412)通過抑制腫瘤血管生成（通過其對VEGF受體酪氨酸激酶的影響）以及直接抑制腫瘤細(xì)胞增殖（通過其對PKCs的影響），可能抑制腫瘤生長。這種抗血管生成作用可能有助于midostaurin(pkc412)所展示的抗轉(zhuǎn)移和廣泛的抗腫瘤活性，以及與包括多柔比星、環(huán)磷酰胺、順鉑和吉西他濱在內(nèi)的細(xì)胞毒性化合物的協(xié)同作用?？诜r，midostaurin(pkc412)的最大耐受劑量為>300 mg/kg。[1]
存儲條件	Powder: -20°C for 3 years \| In solvent: -80°C for 1 year \| Shipping with blue ice.
溶解度	DMSO : 55 mg/mL (96.38 mM)
關(guān)鍵字	CGP-41251 \| Nitric oxide synthases \| SCFR \| inhibit \| CGP-41231 \| Protein kinase C \| Inhibitor \| Apoptosis \| NO Synthase \| CD117 \| Midostaurin \| c-Kit \| VEGFR \| PKC-412 \| NOS \| PKC 412 \| CGP 41231 \| CGP41251 \| Vascular endothelial growth factor receptor \| PKC
相關(guān)產(chǎn)品	Myricitrin \| Chelerythrine chloride \| Mitoxantrone dihydrochloride \| Methyl-Hesperidin \| Fasudil \| Darovasertib \| Staurosporine \| R59949 \| α-Vitamin E \| Ro-3306 \| Daphnetin \| N-Desmethyltamoxifen hydrochloride
相關(guān)庫	經(jīng)典已知活性庫 \| 抗癌上市藥物庫 \| 已知活性化合物庫 \| EMA 上市藥物庫 \| FDA 上市藥物庫 \| 藥物功能重定位化合物庫 \| 抗癌臨床化合物庫 \| 抗癌藥物庫

關(guān)鍵字：米哚妥林|||苯甲?；宙邏A|||PKC412|||N-Benzoylstaurosporine|||CGP41231|||CGP 41251|TargetMol

公司簡介

上海陶術(shù)生物科技有限公司為美國Target Molecule Corp. ( Target Mol ) 在上海建立的全資子公司。我們與美國波士頓、德國慕尼黑的同事一起，為北美、歐洲和亞洲從事藥物研發(fā)和生物學(xué)研究的科學(xué)家提供優(yōu)質(zhì)的產(chǎn)品和專業(yè)的服務(wù)。公司下設(shè)篩選事業(yè)部，化學(xué)事業(yè)部，生物事業(yè)部和新材料部。從虛擬篩選到實體化合物分子供應(yīng)；從商業(yè)化產(chǎn)品銷售到個性化定制合成；從對明確靶點的分子篩選到對明確分子的多靶點篩選，從高通量篩選到化學(xué)結(jié)構(gòu)優(yōu)化，我們都可以滿足您的科研用品及技術(shù)服務(wù)的需求。經(jīng)過在中國市場五年的精心耕耘，我們已成為篩選化合物領(lǐng)域優(yōu)秀的供應(yīng)商，為超過五百家學(xué)校和各類企業(yè)提供了品質(zhì)卓越的小分子化合物和藥物篩

成立日期	2013-04-18 （12年）	注冊資本	566.2651萬人民幣
員工人數(shù)	100-500人	年營業(yè)額	￥ 1億以上
主營行業(yè)	化學(xué)試劑,生物活性小分子	經(jīng)營模式	貿(mào)易,試劑,定制,服務(wù)

TargetMol中國（陶術(shù)生物）

VIP 12年

公司成立：12年
注冊資本：566.2651萬人民幣
企業(yè)類型：有限責(zé)任公司(自然人投資或控股)
主營產(chǎn)品：小分子抑制劑，藥物篩選化合物庫，天然產(chǎn)物，活性分子化合物等
公司地址：上海市閘北區(qū)江場三路28號4樓

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