布雷非德菌素 A|T6062

價格	￥414	￥698	￥2470
包裝	5mg	10mg	50mg

最小起訂量	1mg
發(fā)貨地	上海
更新日期	2024-12-02

QQ交談微信洽談

產(chǎn)品詳情

中文名稱:布雷非德菌素 A	英文名稱:Brefeldin A
CAS:20350-15-6	品牌: TargetMol
產(chǎn)地: 美國	保存條件: store at low temperature \| Powder: -20°C for 3 years \| In solvent: -80°C for 1 year \| Shipping with blue ice.
純度規(guī)格: 99.89%	產(chǎn)品類別: 抑制劑
貨號: T6062

Product Introduction

Bioactivity

名稱	Brefeldin A
描述	Brefeldin A (Cyanein) belongs to the class of macrolide antibiotics and is an ATPase inhibitor (IC50=0.2 μM). Brefeldin A can induce tumor cell differentiation and apoptosis, and also possesses autophagy inhibitory activity.
細(xì)胞實驗	HF1A3, HF4.9 cell viability upon the treatments is tested using double staining of cells with YO-PRO 1/PI and SYTO16/PI probes. To access cell proliferation, cells are treated with 0–100 ng/mL Brefeldin A in complete medium for 20 hours before adding 1 μCi/mL [methyl-3H]-thymidine for additional 4 hours at 37 °C. The incorporated radioactive thymidine is quantified by scintillation counting with Microbeta counter. To examine long-term effects of Brefeldin A treatment, cells are seeded at initial concentration 105 cells/mL and treated with 0-75 ng/mL Brefeldin A for up to 5 days. At the time indicated, a sample of cells is removed and viable cell number is assessed by standard Trypan Blue exclusion assay.(Only for Reference)
激酶實驗	ELISA-based active site binding assay: Samples (lysed cells or tissue homogenates) are treated for 1 h at room temperature with the biotinylated active site probe PR-584 (5-15 μM). Samples are denatured by addition of SDS (0.9% final) and heating to 100 °C for 5 min. The denatured samples are transferred to a 96-well or 384-well filter plat, mixed with streptavidin-sepharose beads (2.5-5 μL packed beads/well), and incubated for 1 h at room temperature on a plate shaker. The beads are washed 5 times with 100-200 μL /well of ELISA buffer (PBS, 1% bovine serum albumin, 0.1% Tween-20) by vacuum filtration. The beads are incubated overnight at 4 °C on a plate shaker with the following antibodies recognizing the six catalytic subunits diluted into ELISA buffer: β5, β1, and β2 diluted 1:3000, LMP7 and LMP2 diluted 1:5000, and MECL-1 diluted 1:1000. The beads are washed 5 times with 100-200 μL /well of ELISA buffer and incubated with HRP-conjugated secondary antibody diluted 1:5000 in ELISA buffer and incubated 2 h at room temperature on a plate shaker. The beads are washed 5 times with 100-200 μL /well of ELISA buffer and developed for chemiluminsecence signal using the supersignal ELISA pico substrate following the manufacturer's instructions. Luminescence is measured on a plate reader and converted to ng of proteasome or μg/ml of lysate by comparison with 20S proteasome or untreated cell lysate standard curves. For proteasome inhibitor studies, active site probe binding values are expressed as the percent of binding relative to DMSO treated cells.
體外活性	方法：腫瘤細(xì)胞 HL60 、K562 和 HT-29 用 Brefeldin A (2 μM) 處理 72 h，使用 DNA filter elution assay 檢測 DNA 片段。結(jié)果：Brefeldin A 以不同的動力學(xué)誘導(dǎo) DNA 斷裂。HL60 細(xì)胞在 15 h 內(nèi)觀察到完整的 DNA 片段，而 K562 和 HT-29 細(xì)胞則需要 48-72 h。[1] 方法：人乳腺癌細(xì)胞 MDA-MB-231 用 Brefeldin A (0.05-1 μg/mL) 處理 24 h，使用 Western Blot 方法檢測靶點蛋白表達(dá)水平。結(jié)果：PARP 切割是細(xì)胞死亡的標(biāo)志性事件，可以在 Brefeldin A 處理的懸浮 MDA-MB-231 細(xì)胞中檢測到。[2]
體內(nèi)活性	方法：為檢測體內(nèi)抗腫瘤活性，將 Brefeldin A (15 mg/kg in 10% ricinus oil+5% DMSO+10% ethanol+75% physiologic saline) 腹腔注射給攜帶人結(jié)直腸癌腫瘤 HCT116 的 BALB/c 小鼠，每天一次，持續(xù)四周。結(jié)果：Brefeldin A 在體內(nèi)抑制 HCT116 腫瘤生長。[3] 方法：為檢測體內(nèi)抗腫瘤活性，將 Brefeldin A (16-64 mg/kg in distilled water containing 0.05% Tween 80) 腹腔注射給攜帶腫瘤 LOX IMVI 的 Athymic NCr nu/nu 小鼠，每天兩次，持續(xù)五天。結(jié)果：Brefeldin A 在體內(nèi)顯示出抗腫瘤活性，小鼠壽命增加 65%-100%，第 60 天幸存者增加 17%-50%。[4]
存儲條件	store at low temperature \| Powder: -20°C for 3 years \| In solvent: -80°C for 1 year \| Shipping with blue ice.
溶解度	Ethanol : 2.8 mg/mL (10 mM) DMSO : 14 mg/mL (50 mM)
關(guān)鍵字	Antibiotic \| Autophagy \| Inhibitor \| CRISPR/Cas9 \| inhibit \| Mitophagy \| HSV \| Herpes simplex virus \| Mitochondrial Autophagy \| Brefeldin A
相關(guān)產(chǎn)品	Guanidine hydrochloride \| Naringin \| Valproic Acid \| Doxycycline \| Neomycin sulfate \| Hydroxychloroquine \| Stavudine \| Ampicillin sodium \| Sulfamethoxazole sodium \| Paeonol \| Kanamycin sulfate \| Sodium 4-phenylbutyrate
相關(guān)庫	抑制劑庫 \| 經(jīng)典已知活性庫 \| 抗癌活性化合物庫 \| 離子通道庫 \| 中藥單體化合物庫 \| 膜蛋白靶向化合物庫 \| 抗病毒中藥單體化合物庫 \| 中藥抗炎分子庫 \| 抗感染天然產(chǎn)物庫

關(guān)鍵字：布雷非德菌素 A|||Decumbin|||Cyanein|||BFA|||Ascotoxin|TargetMol

公司簡介

上海陶術(shù)生物科技有限公司為美國Target Molecule Corp. ( Target Mol ) 在上海建立的全資子公司。我們與美國波士頓、德國慕尼黑的同事一起，為北美、歐洲和亞洲從事藥物研發(fā)和生物學(xué)研究的科學(xué)家提供優(yōu)質(zhì)的產(chǎn)品和專業(yè)的服務(wù)。公司下設(shè)篩選事業(yè)部，化學(xué)事業(yè)部，生物事業(yè)部和新材料部。從虛擬篩選到實體化合物分子供應(yīng)；從商業(yè)化產(chǎn)品銷售到個性化定制合成；從對明確靶點的分子篩選到對明確分子的多靶點篩選，從高通量篩選到化學(xué)結(jié)構(gòu)優(yōu)化，我們都可以滿足您的科研用品及技術(shù)服務(wù)的需求。經(jīng)過在中國市場五年的精心耕耘，我們已成為篩選化合物領(lǐng)域優(yōu)秀的供應(yīng)商，為超過五百家學(xué)校和各類企業(yè)提供了品質(zhì)卓越的小分子化合物和藥物篩

成立日期	2013-04-18 （12年）	注冊資本	566.2651萬人民幣
員工人數(shù)	100-500人	年營業(yè)額	￥ 1億以上
主營行業(yè)	化學(xué)試劑,生物活性小分子	經(jīng)營模式	貿(mào)易,試劑,定制,服務(wù)

TargetMol中國（陶術(shù)生物）

VIP 12年

公司成立：12年
注冊資本：566.2651萬人民幣
企業(yè)類型：有限責(zé)任公司(自然人投資或控股)
主營產(chǎn)品：小分子抑制劑，藥物篩選化合物庫，天然產(chǎn)物，活性分子化合物等
公司地址：上海市閘北區(qū)江場三路28號4樓

進(jìn)入店鋪

詢盤

店內(nèi)推薦

上海陶素獨家代理chemdiv成藥庫

￥100000

雷帕霉素

￥286.00

雷帕霉素

￥396