Human Hyaluronidase PH-20 / SPAM1 Protein, Tag Free (active enzyme, MALS verified)
表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）
Human Hyaluronidase PH-20 Protein, Tag Free (PH0-H5219) is expressed from human 293 cells (HEK293). It contains AA Leu 36 - Ser 490 (Accession # P38567-1).
Predicted N-terminus: Leu 36
蛋白結(jié)構(gòu)（Molecular Characterization）
This protein carries no "tag".
The protein has a calculated MW of 52.6 kDa. The protein migrates as 65-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
內(nèi)毒素（Endotoxin）
Less than 0.1 EU per μg by the LAL method.
純度（Purity）
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
制劑（Formulation）
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
重構(gòu)方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存儲（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
背景（Background）
透明質(zhì)酸酶PH-20也稱為精子粘附分子1（SPAM1）和精子表面蛋白PH-20，屬于糖基水解酶56家族，SPAM1/PH-20在睪丸中表達(dá)。透明質(zhì)酸中N-乙酰基-β-D-葡糖胺和D-葡萄糖醛酸殘基之間（1->4）鍵的SPAM-1/PH20隨機(jī)水解。SPAM-1/PH20參與精卵粘附。受精后，精子必須首先穿透卵子周圍的一層卵丘細(xì)胞，然后才能到達(dá)透明帶。卵丘細(xì)胞包埋在排卵前形成的含有透明質(zhì)酸的基質(zhì)中。SPAM1通過消化透明質(zhì)酸幫助穿透卵丘細(xì)胞層。
關(guān)鍵字： Hyaluronidase;人透明質(zhì)酸酶;透明質(zhì)酸酶PH20;ACRO;百普賽斯;
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