Human CD73 / NT5E Protein, His Tag (HPLC-verified) (active enzyme)
分子別名(Synonym)
CD73,NT5E,5'-Nucleotidase,5'-NT,NT5,NTE
表達(dá)區(qū)間及表達(dá)系統(tǒng)(Source)
Human CD73 Protein, His Tag (CD3-H52H7) is expressed from human 293 cells (HEK293). It contains AA Trp 27 - Ser 549 (Accession # P21589-1).
Predicted N-terminus: Trp 27
蛋白結(jié)構(gòu)(Molecular Characterization)
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 59.9 kDa. The protein migrates as 65 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
內(nèi)毒素(Endotoxin)
Less than 1.0 EU per μg by the LAL method.
純度(Purity)
>90% as determined by SDS-PAGE.
>90% as determined by SEC-HPLC.
制劑(Formulation)
Lyophilized from 0.22 μm filtered solution in 20 mM Tris, 120 mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
重構(gòu)方法(Reconstitution)
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存儲(chǔ)(Storage)
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 24 months in lyophilized state;
-70°C for 12 months under sterile conditions after reconstitution.
背景(Background)
5'-核苷酸酶(5'-NT),也稱為胞外-5'-核苷酸酶或CD73(分化簇73),是由NT5E基因編碼的酶。CD73通常用于將AMP轉(zhuǎn)化為腺苷。外切5-三核苷酸酶(5-核糖核苷酸磷酸水解酶)在中性pH下催化嘌呤5-三核苷酸轉(zhuǎn)化為核苷,優(yōu)選的底物是AMP。其他形式的5-三聚核苷酸酶存在于細(xì)胞質(zhì)和溶酶體中,可以通過其底物親和力、對(duì)二價(jià)鎂離子的需求、ATP的激活和無機(jī)磷酸鹽的抑制與胞外NPT5區(qū)分開來。罕見的等位基因變異與成人發(fā)病的關(guān)節(jié)和動(dòng)脈鈣化綜合征(CALJA)有關(guān),該綜合征影響髂、股和脛動(dòng)脈,減少腿部和手腳關(guān)節(jié)的循環(huán),導(dǎo)致疼痛。
關(guān)鍵字: CD73;CD73蛋白;NT5E蛋白;ACRO;百普賽斯;
百普賽斯集團(tuán)ACROBiosystems Group(股票代碼:301080)是成立于2010年的跨國生物科技公司,是為全球生物醫(yī)藥、健康產(chǎn)業(yè)領(lǐng)域提供關(guān)鍵生物試劑產(chǎn)品及解決方案的行業(yè)平臺(tái)型基石企業(yè)。2021年在創(chuàng)業(yè)板上市。百普賽斯集團(tuán)業(yè)務(wù)遍布全球,橫跨亞洲、北美洲、歐洲,在中國、美國、瑞士等12個(gè)城市設(shè)有辦公室、研發(fā)中心及生產(chǎn)基地。目前累計(jì)服務(wù)客戶超6000家,與全球Top 20醫(yī)藥企業(yè)均建立了長期、穩(wěn)定的合作伙伴關(guān)系。集團(tuán)旗下?lián)碛衅放艫CROBiosystems百普賽斯、bioSeedin柏思薈、Condense Capital墾拓資本和ACRODiagnostics百斯醫(yī)學(xué)等。