Human 4-1BB Ligand / TNFSF9 (71-254) Protein, His,Flag Tag, active trimer (MALS verified)

分子別名（Synonym）

4-1BB Ligand,TNFSF9,CD137L

表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

Human 4-1BB Ligand (71-254) Protein, His,Flag Tag (41L-H52D4) is expressed from human 293 cells (HEK293). It contains AA Arg 71 - Glu 254 (Accession # P41273-1).

Predicted N-terminus: His

蛋白結(jié)構(gòu)（Molecular Characterization）

This protein carries a polyhistidine tag at the N-terminus, followed by a flag tag.

The protein has a calculated MW of 62.8 kDa. The protein migrates as 55-60 kDa under non-reducing (NR) condition (SDS-PAGE).

內(nèi)毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

純度（Purity）

>95% as determined by SDS-PAGE.

制劑（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重構(gòu)方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲(chǔ)（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

腫瘤壞死因子配體超家族成員9（4-1BBL）也稱(chēng)為4-1BB配體、CD137L或TNFSF9，它是一種與TNFRSF9結(jié)合的細(xì)胞因子。4-1BBL是4-1BB的高親和力配體。4-1BBL誘導(dǎo)活化的外周血T細(xì)胞增殖。此外，4-1BBL可能在活化誘導(dǎo)的細(xì)胞死亡（AICD）中發(fā)揮作用。此外，4-1BBL可能在T細(xì)胞和B細(xì)胞/巨噬細(xì)胞之間的同源相互作用中發(fā)揮作用。至于疾病，4-1BBL與癌癥、傳染病和自身免疫性疾病有關(guān)。