坦螺旋霉素|T6290

價格	￥514.00	￥558.00	￥359.00	￥919.00	￥1450.00
包裝	1瓶	10瓶	5瓶	25瓶	50瓶

最小起訂量	1瓶
發(fā)貨地	上海
更新日期	2024-12-02

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產(chǎn)品詳情

中文名稱:坦螺旋霉素	英文名稱:Tanespimycin
CAS:75747-14-7	品牌: TargetMol
產(chǎn)地: 美國	保存條件: Powder: -20°C for 3 years \| In solvent: -80°C for 1 year \| Shipping with blue ice.
純度規(guī)格: 99.83%	產(chǎn)品類別: 抑制劑
貨號: T6290

Product Introduction

Bioactivity

名稱	Tanespimycin
描述	Tanespimycin (KOS 953) (17-AAG) is an inhibitor of Hsp90 that selectively inhibits BT474 tumor cell Hsp90 (IC50: 5 nM).
細胞實驗	Cells were seeded in 96-well plates at 2,000 cells per well in a final culture volume of 100 μl for 24 h before the addition of increasing concentrations of 17-AAG that was incubated for 5 days. Viable cell number was determined using the Celltiter 96 AQueous Nonradioactive Cell Proliferation Assay. The value of the background absorbance at 490 nm (A490) of wells not containing cells was subtracted. Percentage of viable cells ? (A490 of 17-AAG treated sample/A490 untreated cells) × 100. The IC50 was defined as the concentration that gave rise to 50% viable cell number [1].
激酶實驗	Purified native Hsp90 protein or cell lysates in lysis buffer (20 mM HEPES, pH 7.3, 1 mM EDTA, 5 mM MgCl2, 100 mM KCl) were incubated with or without 17-AAG for 30 min at 4 °C, and then incubated with biotin-GM linked to streptavidin magnetic beads for 1 h at 4 °C. Tubes were placed on a magnetic rack, and the unbound supernatant removed. The magnetic beads were washed three times in lysis buffer and heated for 5 min at 95 °C in SDS–PAGE sample buffer. Samples were analyzed on SDS protein gels, and western blots done using indicated antibodies. Bands in the western blots were quantified, and the percentage inhibition of binding of Hsp90 to the biotin-GM was calculated. The IC50 reported is the concentration of 17-AAG needed to cause half-maximal inhibition of binding. For in vitro reconstitution, 5 μM of purified Hsp90 was combined with 1 μM each of Hsp70, Hsp40, p23, and Hop purified proteins [1].
動物實驗	B10.BR mice were inoculated with 5×10^5 lymphoma cells through intraperitoneal injection. Seven days following tumor implantation, the mice were I.P. injected with 17-AAG or vehicle (10% DMSO + 40% Cremophor EL: Ethanol (3:1) (v/v) + 50 % PBS) every other day for three weeks. At the cessation of treatment, mice were monitored up to 80 days post tumor cell injection. To determine the effects of 17-AAG on lymphoma initiation in vivo, secondary B10.BR recipient mice were implanted by intraperitoneal injection of 1×10^5 lymphoma cells from the spleens of first-round mice that had been treated with 17-AAG or vehicle. These mice were followed up to 160 days post tumor cell injection to monitor differences in tumor initiation between the mice [4].
體外活性	源自腫瘤細胞的Hsp90與Tanespimycin的結(jié)合親和力是正常細胞中Hsp90的100倍。體外重組Hsp90伴侶復合物后，其與Tanespimycin的結(jié)合親和力增強，且ATP酶活性增加[1]。Tanespimycin能促進HER2、Akt以及突變型和野生型AR的降解，并導致依賴視網(wǎng)膜母細胞瘤的G1期生長阻滯在前列腺癌細胞中[2]。Tanespimycin與Trastuzumab聯(lián)合處理ErbB2過表達的乳腺癌細胞系，增強了ErbB2的泛素化、從細胞表面的下調(diào)及溶酶體降解[3]。
體內(nèi)活性	在非毒性劑量下，Tanespimycin導致前列腺癌異種移植瘤中AR、HER2和Akt表達水平呈劑量依賴性下降。這種下降速度很快，HER2和AR表達在4小時內(nèi)分別下降了97%和80% [2]。相比之下，接受了Tanespimycin（5至40 mg/kg）治療的小鼠的脾臟明顯變小，脾臟中滲透的淋巴瘤細胞減少，轉(zhuǎn)移至其他器官的癌細胞也顯著降低，與接受車輛對照組治療的小鼠相比。此外，接受Tanespimycin治療的小鼠比僅接受車輛處理的小鼠存活時間顯著延長 [4]。
存儲條件	Powder: -20°C for 3 years \| In solvent: -80°C for 1 year \| Shipping with blue ice.
溶解度	DMSO : 22.5 mg/mL (38.42 mM), Sonication is recommended.
關(guān)鍵字	tumor \| stk38 \| Inhibitor \| KOS953 \| cancer \| prostate \| HSP \| Heat shock proteins \| Mitophagy \| NSC-330507 \| Mitochondrial Autophagy \| Tanespimycin \| Apoptosis \| CP127374 \| inhibit \| Autophagy \| CP-127374 \| HER2 \| KOS-953 \| A549 \| NSC330507 \| Bacterial \| Antibiotic
相關(guān)產(chǎn)品	Guanidine hydrochloride \| Doxycycline \| Neomycin sulfate \| Hydroxychloroquine \| Dimethyl sulfoxide \| Sulfamethoxazole sodium \| Paeonol
相關(guān)庫	抑制劑庫 \| 微生物天然產(chǎn)物庫 \| 經(jīng)典已知活性庫 \| 已知活性化合物庫 \| 激酶抑制劑庫 \| 抗衰老化合物庫 \| 高選擇性抑制劑庫 \| 藥物功能重定位化合物庫 \| 抗癌臨床化合物庫 \| 抗癌藥物庫

關(guān)鍵字： 17-AAG|||CP 127374|||NSC 330507|||KOS 953|||坦螺旋霉素

公司簡介

上海陶術(shù)生物科技有限公司為美國Target Molecule Corp. ( Target Mol ) 在上海建立的全資子公司。我們與美國波士頓、德國慕尼黑的同事一起，為北美、歐洲和亞洲從事藥物研發(fā)和生物學研究的科學家提供優(yōu)質(zhì)的產(chǎn)品和專業(yè)的服務。公司下設篩選事業(yè)部，化學事業(yè)部，生物事業(yè)部和新材料部。從虛擬篩選到實體化合物分子供應；從商業(yè)化產(chǎn)品銷售到個性化定制合成；從對明確靶點的分子篩選到對明確分子的多靶點篩選，從高通量篩選到化學結(jié)構(gòu)優(yōu)化，我們都可以滿足您的科研用品及技術(shù)服務的需求。經(jīng)過在中國市場五年的精心耕耘，我們已成為篩選化合物領(lǐng)域優(yōu)秀的供應商，為超過五百家學校和各類企業(yè)提供了品質(zhì)卓越的小分子化合物和藥物篩

成立日期	2013-04-18 （12年）	注冊資本	566.2651萬人民幣
員工人數(shù)	100-500人	年營業(yè)額	￥ 1億以上
主營行業(yè)	化學試劑,生物活性小分子	經(jīng)營模式	貿(mào)易,試劑,定制,服務

TargetMol中國（陶術(shù)生物）

VIP 12年

公司成立：12年
注冊資本：566.2651萬人民幣
企業(yè)類型：有限責任公司(自然人投資或控股)
主營產(chǎn)品：小分子抑制劑，藥物篩選化合物庫，天然產(chǎn)物，活性分子化合物等
公司地址：上海市閘北區(qū)江場三路28號4樓

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