Human IL-31 Protein, His Tag (MALS verified)

分子別名（Synonym）

IL-31,IL31,Interleukin-31

表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

Human IL-31, His Tag (IL1-H5247) is expressed from human 293 cells (HEK293). It contains AA Ser 24 - Thr 164 (Accession # Q6EBC2-1).

Predicted N-terminus: His

蛋白結(jié)構(gòu)（Molecular Characterization）

This protein carries a polyhistidine tag at the N-terminus.

The protein has a calculated MW of 17.7 kDa. The protein migrates as 22-25 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

內(nèi)毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

純度（Purity）

>90% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制劑（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重構(gòu)方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

IL-31是一種炎性細(xì)胞因子，有助于引發(fā)針對病原體的細(xì)胞介導(dǎo)免疫。通過由IL31RA和OSMR組成的IL31異二聚體受體激活STAT3，并可能激活STAT1和STAT5。它也被確定為包括特應(yīng)性皮炎在內(nèi)的許多慢性炎癥性疾病的主要參與者?？赡軐ζつw免疫起作用。IL-31由多種細(xì)胞產(chǎn)生，即2型輔助（TH2）T細(xì)胞。IL-31通過由IL-31RA和在免疫細(xì)胞和上皮細(xì)胞中表達(dá)的抑瘤素M受體β（OSMRβ）組成的受體復(fù)合物發(fā)送信號。這些信號激活三條通路：ERK1/2 MAP激酶、PI3K/AKT和JAK1/2信號通路。