Human Integrin alpha V beta 5 (ITGAV&ITGB5) Heterodimer Protein, His Tag&Tag Free
分子別名（Synonym）
Integrin alpha V beta 5,ITGAV&ITGB5
表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）
Human ITGAV&ITGB5 Heterodimer Protein, His Tag&Tag Free (IT5-H52W5) is expressed from human 293 cells (HEK293). It contains AA Phe 31 - Val 992 (ITGAV) & Gly 24 - Asn 719 (ITGB5) (Accession # NP_002201.1 (ITGAV) & NP_002204.2 (ITGB5)).
Predicted N-terminus: Phe 31 (ITGAV) & Gly 24 (ITGB5)
蛋白結(jié)構(gòu)（Molecular Characterization）
Human ITGAV&ITGB5 Heterodimer Protein, His Tag&Tag Free, produced by co-expression of ITGAV and ITGB5, has a calculated MW of 112.9 kDa (ITGAV) and 81.9 kDa (ITGB5). Subunit ITGAV is fused with polyhistidine tag at the C-terminus and followed by a acidic tail and subunit ITGB5 contains no tag but a basic tail at the C-terminus. The non-reducing (NR) protein migrates as 130-150 kDa (ITGAV) and 85-95 kDa (ITGB5) respectively due to glycosylation.
內(nèi)毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
純度（Purity）
>95% as determined by SDS-PAGE.
制劑（Formulation）
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
重構(gòu)方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存儲(chǔ)（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
背景（Background）
整合素αVβ5（ITGAV和ITGB5）在多種細(xì)胞類型上表達(dá)，包括角質(zhì)形成細(xì)胞、成纖維細(xì)胞、粘附性單核細(xì)胞、胚胎干細(xì)胞以及特定的內(nèi)皮和上皮細(xì)胞。ITGAV和ITGB5結(jié)合含有RGD基序的配體，特別是玻連蛋白。增加PKC活性的生長(zhǎng)因子，如VEGF或TGF-α，促進(jìn)ITGAV和ITGB5介導(dǎo)的血管生成，而可能在同一細(xì)胞中表達(dá)的αVβ3對(duì)FGF堿性和TNF-α有反應(yīng)。兩者的抑制劑都能下調(diào)腫瘤血管生成。在肺部炎癥期間，肌成纖維細(xì)胞或浸潤(rùn)淋巴細(xì)胞上ITGAV和ITGB5的上調(diào)可能通過(guò)釋放TGF-β來(lái)促進(jìn)纖維化。
關(guān)鍵字： Integrin αvβ5;整合素αvβ5;αvβ5;ACRO;百普賽斯;
百普賽斯集團(tuán)ACROBiosystems Group（股票代碼：301080）是成立于2010年的跨國(guó)生物科技公司，是為全球生物醫(yī)藥、健康產(chǎn)業(yè)領(lǐng)域提供關(guān)鍵生物試劑產(chǎn)品及解決方案的行業(yè)平臺(tái)型基石企業(yè)。2021年在創(chuàng)業(yè)板上市。百普賽斯集團(tuán)業(yè)務(wù)遍布全球，橫跨亞洲、北美洲、歐洲，在中國(guó)、美國(guó)、瑞士等12個(gè)城市設(shè)有辦公室、研發(fā)中心及生產(chǎn)基地。目前累計(jì)服務(wù)客戶超6000家，與全球Top 20醫(yī)藥企業(yè)均建立了長(zhǎng)期、穩(wěn)定的合作伙伴關(guān)系。集團(tuán)旗下?lián)碛衅放艫CROBiosystems百普賽斯、bioSeedin柏思薈、Condense Capital墾拓資本和ACRODiagnostics百斯醫(yī)學(xué)等。