Human gp130 / CD130 / IL-6 R beta Protein, His Tag (MALS verified)
表達(dá)區(qū)間及表達(dá)系統(tǒng)(Source)
Human gp130, His Tag (ILT-H52H2) is expressed from human 293 cells (HEK293). It contains AA Glu 23 - Glu 619 (Accession # P40189-1).
Predicted N-terminus: Glu 23
蛋白結(jié)構(gòu)(Molecular Characterization)
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 69.6 kDa. The protein migrates as 80-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
內(nèi)毒素(Endotoxin)
Less than 1.0 EU per μg by the LAL method.
純度(Purity)
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制劑(Formulation)
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重構(gòu)方法(Reconstitution)
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存儲(chǔ)(Storage)
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
背景(Background)
白細(xì)胞介素-6受體亞基β(IL6ST)也稱為IL-6受體亞基α、IL-6R亞基β、IL-6Rβ、IL-6RB、白細(xì)胞介蛋白-6信號(hào)轉(zhuǎn)導(dǎo)子、膜糖蛋白130(gp130)、CD130、Oncostatin-M受體亞基a和IL6ST,后者是單程I型膜蛋白。IL6ST/gp130/CD130可以在腦、心臟、胸腺、脾臟、腎臟、肺和肝臟等組織中發(fā)現(xiàn),并且在除BaF-B03之外的所有測試細(xì)胞系中都有發(fā)現(xiàn)。IL-6ST/gp130的表達(dá)不限于IL6反應(yīng)細(xì)胞。IL6、LIF、OSM、CNTF、IL11、CTF1和BSF3的受體系統(tǒng)可以利用gp130啟動(dòng)信號(hào)傳輸。IL6ST/CD130可以與IL6/IL6R(α鏈)復(fù)合物結(jié)合,形成高親和力IL6結(jié)合位點(diǎn),并轉(zhuǎn)導(dǎo)信號(hào)。IL6ST/GP130不結(jié)合IL6,可能在胚胎發(fā)育中發(fā)揮作用。
關(guān)鍵字: gp130;gp130蛋白;gp130重組蛋白;ACRO;百普賽斯;
百普賽斯集團(tuán)ACROBiosystems Group(股票代碼:301080)是成立于2010年的跨國生物科技公司,是為全球生物醫(yī)藥、健康產(chǎn)業(yè)領(lǐng)域提供關(guān)鍵生物試劑產(chǎn)品及解決方案的行業(yè)平臺(tái)型基石企業(yè)。2021年在創(chuàng)業(yè)板上市。百普賽斯集團(tuán)業(yè)務(wù)遍布全球,橫跨亞洲、北美洲、歐洲,在中國、美國、瑞士等12個(gè)城市設(shè)有辦公室、研發(fā)中心及生產(chǎn)基地。目前累計(jì)服務(wù)客戶超6000家,與全球Top 20醫(yī)藥企業(yè)均建立了長期、穩(wěn)定的合作伙伴關(guān)系。集團(tuán)旗下?lián)碛衅放艫CROBiosystems百普賽斯、bioSeedin柏思薈、Condense Capital墾拓資本和ACRODiagnostics百斯醫(yī)學(xué)等。