PE-Labeled Monoclonal Anti-G4S linker Antibody, Rabbit IgG (016) (0.03% Proclin)

抗體來(lái)源（Source）

Monoclonal Anti-G4S linker Antibody, Rabbit IgG (016) is a rabbit monoclonal antibody recombinantly expressed from human 293 cells (HEK293)，which provides higher batch consistency and long term security of supply. It shows superior performance in flow cytometry assays with higher affinity and better specificity.

應(yīng)用（Application）

Flow Cytometry (Evaluation of cell surface expressed CARs of varying specificity containing a G4S linker within the scFv of the extracellular domain).

克隆號(hào)（Clone）

016

種屬（Species）

Rabbit

亞型（Isotype）

Rabbit IgG | Rabbit Kappa

特異性（Specificity）

Specifically recognizes the scFv-based CARs containing a G4S linker.

偶聯(lián)（Conjugate）

PE

Excitation Wavelength: 488 nm / 561 nm

Emission Wavelength: 575 nm

推薦稀釋比（Recommended Dilution）

1:50

制劑（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, 0.03% Proclin 300, pH7.4, 0.2% BSA with trehalose as protectant.

Contact us for customized product form or formulation.

重構(gòu)方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲(chǔ)（Storage）

Please protect from light and avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 24 months in lyophilized state;

-70°C for 12 months after reconstitution.

2-8°C for 12 months after reconstitution.

背景（Background）

G4S linker是單鏈抗體最常見(jiàn)的連接肽。它由重復(fù)序列（Gly-Gly-Gly-Gly-Ser）組成，通常在scFv片段中以15-mer（G4S）3或20-mer（G4S）4的形式存在。G4S linker已成為絕大多數(shù)CAR不可或缺的一部分，因?yàn)樗B接scFvs的VH和VL識(shí)別結(jié)構(gòu)域。G4S linker通常用于靶向?qū)嶓w瘤抗原（如HER2和CEA）的CAR，而惠特洛/218接頭肽主要用于靶向血液系統(tǒng)惡性腫瘤（如CD19 CAR產(chǎn)物）的CAR構(gòu)建。