Human BDNF / Abrineurin Protein, premium grade

分子別名（Synonym）

BDNF,ANON2,BULN2,Abrineurin

表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

Human BDNF, premium grade (BDF-H5219) is expressed from human 293 cells (HEK293). It contains AA His 129 - Arg 247 (Accession # P23560-1).

Predicted N-terminus: His 129

該產(chǎn)品是在我們嚴(yán)格的質(zhì)量控制體系下生產(chǎn)的，該體系包含一套全面的測試，包括無菌和內(nèi)毒素測試。在早期臨床前階段，產(chǎn)品性能經(jīng)過仔細(xì)驗(yàn)證和測試，以確保其與細(xì)胞培養(yǎng)用途或任何其他應(yīng)用的兼容性。當(dāng)準(zhǔn)備過渡到后期臨床階段時(shí)，我們還提供定制的GMP蛋白質(zhì)服務(wù)，以滿足您的需求。我們將與您合作，根據(jù)您的要求定制和開發(fā)GMP級(jí)產(chǎn)品，該產(chǎn)品也符合細(xì)胞療法細(xì)胞制造中使用的原材料和輔助材料的要求。

蛋白結(jié)構(gòu)（Molecular Characterization）

This protein carries no "tag".

The protein has a calculated MW of 13.5 kDa. The protein migrates as 13-15 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

內(nèi)毒素（Endotoxin）

Less than 0.01 EU per μg by the LAL method.

無菌（Sterility）

Negative

支原體（Mycoplasma）

Negative.

純度（Purity）

>95% as determined by SDS-PAGE.

制劑（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重構(gòu)方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲(chǔ)（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

腦源性神經(jīng)營養(yǎng)因子（BDNF）與神經(jīng)生長因子（NGF）、神經(jīng)營養(yǎng)因子-3（NT-3）和神經(jīng)營養(yǎng)因子-4/5（NT-4/5）一起是神經(jīng)營養(yǎng)因子家族的成員。神經(jīng)營養(yǎng)因子在外周和中樞神經(jīng)系統(tǒng)中各種神經(jīng)元的發(fā)育、存活和功能中起著至關(guān)重要的作用。神經(jīng)營養(yǎng)因子與兩種細(xì)胞表面受體相互作用，即低親和力P75受體和高親和力酪氨酸激酶受體Trk家族。NGF優(yōu)先結(jié)合TrkA，BDNF和NT4/5結(jié)合TrkB，NT-3結(jié)合TrkC（和TrkA的結(jié)合程度較?。?。