Human CD39 / ENTPD1 Protein, His Tag (active enzyme, MALS verified)

分子別名（Synonym）

CD39,ENTPD1,NTPDase 1,Entpd1,Ecto-ATPDase 1,Ecto-ATPase 1

表達區(qū)間及表達系統(tǒng)（Source）

Human CD39, His Tag (CD9-H52H4) is expressed from human 293 cells (HEK293). It contains AA Thr 38 - Val 478 (Accession # P49961-1).

蛋白結(jié)構(gòu)（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 52.3 kDa. The protein migrates as 65-80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

內(nèi)毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

純度（Purity）

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制劑（Formulation）

Lyophilized from 0.22 μm filtered solution in Tris and NaCl, pH8.0 with trehalose as protectant.

Contact us for customized product form or formulation.

重構(gòu)方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

CD39在神經(jīng)系統(tǒng)中也被稱為外核苷三磷酸二磷酸水解酶1、ENTPD1、NTPDase 1、外ATP酶1，可以水解ATP和其他核苷酸來調(diào)節(jié)嘌呤能神經(jīng)傳遞。也可能通過將血小板活化ADP水解為AMP來預(yù)防血小板聚集。水解ATP和ADP同樣好。NTPDase-1最初被描述為CD39，一種B淋巴細胞表面標(biāo)志物，但它也存在于自然殺傷細胞、T細胞和一些內(nèi)皮細胞的表面。調(diào)節(jié)性T細胞（Tregs）通過多種非冗余、細胞接觸依賴和獨立的機制介導(dǎo)免疫抑制，越來越多的證據(jù)表明CD39-CD73-腺苷途徑起著重要作用。CD39外核苷酸酶是級聯(lián)反應(yīng)的限速酶，導(dǎo)致抑制性腺苷的產(chǎn)生，從而改變CD4和CD8 T細胞以及自然殺傷細胞的抗腫瘤活性。