Biotinylated Human LILRB1 / CD85j / ILT2 Protein, Fc,Avitag?

分子別名（Synonym）

CD85J,LILRB1,CD85,ILT2,LIR1,MIR7

表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

Biotinylated Human LILRB1, Fc,Avitag (CDJ-H82F7) is expressed from human 293 cells (HEK293). It contains AA Gly 24 - His 458 (Accession # D9IDM8-1).

Predicted N-terminus: Gly 24

蛋白結(jié)構(gòu)（Molecular Characterization）

This protein carries a human IgG1 Fc tag at the C-terminus, followed by a Avi tag (Avitag?).

The protein has a calculated MW of 75.6 kDa. The protein migrates as 100-116 kDa under reducing (R) condition, and 150 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.

標(biāo)記（Labeling）

Biotinylation of this product is performed using Avitag? technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白標(biāo)記度（Protein Ratio）

Passed as determined by the HABA assay / binding ELISA.

內(nèi)毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

純度（Purity）

>95% as determined by SDS-PAGE.

制劑（Formulation）

Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.

Contact us for customized product form or formulation.

重構(gòu)方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲(chǔ)（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

LILRB1（Uniprot No.D9IDM8-1）是一種與NK細(xì)胞上LILRB1低表達(dá)相關(guān)的變體。它反映了LILRB1基因座的多樣性及其對(duì)NK細(xì)胞表達(dá)模式的影響。LILRB1屬于LIR受體的B亞家族，其包含兩個(gè)或四個(gè)細(xì)胞外免疫球蛋白結(jié)構(gòu)域、一個(gè)跨膜結(jié)構(gòu)域和兩到四個(gè)細(xì)胞質(zhì)免疫受體酪氨酸基抑制基序（ITIM）。受體在免疫細(xì)胞上表達(dá)，與抗原呈遞細(xì)胞上的MHC I類分子結(jié)合，并傳遞抑制免疫反應(yīng)刺激的負(fù)信號(hào)。它被認(rèn)為可以控制炎癥反應(yīng)和細(xì)胞毒性，以幫助集中免疫反應(yīng)并限制自身反應(yīng)性。