Human DPPIV / CD26 Protein, His Tag, active dimer (active enzyme, MALS verified)

分子別名（Synonym）

DPP4,ADABP,ADCP2,CD26,DPPIV,TP103

表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

Human DPPIV, His Tag (DP4-H5221) is expressed from human 293 cells (HEK293). It contains AA Asp 34 - Pro 766 (Accession # NP_001926.2).

Predicted N-terminus: Asp 34

蛋白結(jié)構(gòu)（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 85.7 kDa. The protein migrates as 95 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

內(nèi)毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

純度（Purity）

>95% as determined by SDS-PAGE.

>95% as determined by SEC-MALS.

制劑（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重構(gòu)方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲(chǔ)（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

二肽基肽酶IV（DPPIV）也稱為腺苷脫氨酶復(fù)合蛋白2，DPPIV或CD26是大多數(shù)細(xì)胞類型表面表達(dá)的抗原酶，與免疫調(diào)節(jié)、信號(hào)轉(zhuǎn)導(dǎo)和凋亡有關(guān)。它是一種內(nèi)在的膜糖蛋白和絲氨酸外肽酶，從多肽的N端切割X-脯氨酸二肽。DPPIV的底物是含脯氨酸（或丙氨酸）的肽，包括生長(zhǎng)因子、趨化因子、神經(jīng)肽和血管活性肽。DPPIV在葡萄糖代謝中起著重要作用。它負(fù)責(zé)腸促胰島素如GLP-1的降解。DPPIV在腫瘤生物學(xué)中起著重要作用，可用作各種癌癥的標(biāo)志物，其在細(xì)胞表面或血清中的水平在某些腫瘤中升高，在另一些腫瘤中降低。DPPIV還特異性地和高親和力地結(jié)合腺苷脫氨酶。這種互動(dòng)的意義尚未確定。