Biotinylated Human Siglec-9 Protein, His,Avitag?
分子別名(Synonym)
Siglec-9,SIGLEC9,CDw329,CD329
表達區(qū)間及表達系統(tǒng)(Source)
Biotinylated Human Siglec-9, His,Avitag (SI9-H82E9) is expressed from human 293 cells (HEK293). It contains AA Gln 18 - Gly 348 (Accession # Q9Y336-1).
Predicted N-terminus: Gln 18
蛋白結(jié)構(gòu)(Molecular Characterization)
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag?).
The protein has a calculated MW of 39.6 kDa. The protein migrates as 60-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
標記(Biotinylation)
Biotinylation of this product is performed using Avitag? technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
蛋白標記度(Biotin:Protein Ratio)
Passed as determined by the HABA assay / binding ELISA.
內(nèi)毒素(Endotoxin)
Less than 1.0 EU per μg by the LAL method.
純度(Purity)
>95% as determined by SDS-PAGE.
制劑(Formulation)
Lyophilized from 0.22 μm filtered solution in 25 mM MES, 150 mM NaCl, pH5.5. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
重構(gòu)方法(Reconstitution)
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存儲(Storage)
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
背景(Background)
Siglec-9(HGMW批準的符號SIGLEC9)是屬于免疫球蛋白超家族(IgSF)的唾液酸結(jié)合Ig樣凝集素(siglec)家族的成員。SIGLEC9與siglec家族的許多成員顯示出高度的同源性,包括siglec-7(80%)、siglec-8(72%)、siglec-5(65%)和CD33(64%)。這種高度同源性在細胞外Ig樣結(jié)構(gòu)域中也是保守的。它們的特征是N端的Ig樣V型結(jié)構(gòu)域介導(dǎo)唾液酸結(jié)合,隨后是不同數(shù)量的Ig樣C2型結(jié)構(gòu)域Siglec-9具有疏水信號肽、N端Ig樣V型結(jié)構(gòu)域、兩個Ig樣C2型結(jié)構(gòu)域,一個跨膜區(qū)和一個細胞質(zhì)尾部。
關(guān)鍵字: Siglec-9;Siglec-9蛋白;Siglec-9重組蛋白;ACRO;百普賽斯;
百普賽斯集團ACROBiosystems Group(股票代碼:301080)是成立于2010年的跨國生物科技公司,是為全球生物醫(yī)藥、健康產(chǎn)業(yè)領(lǐng)域提供關(guān)鍵生物試劑產(chǎn)品及解決方案的行業(yè)平臺型基石企業(yè)。2021年在創(chuàng)業(yè)板上市。百普賽斯集團業(yè)務(wù)遍布全球,橫跨亞洲、北美洲、歐洲,在中國、美國、瑞士等12個城市設(shè)有辦公室、研發(fā)中心及生產(chǎn)基地。目前累計服務(wù)客戶超6000家,與全球Top 20醫(yī)藥企業(yè)均建立了長期、穩(wěn)定的合作伙伴關(guān)系。集團旗下?lián)碛衅放艫CROBiosystems百普賽斯、bioSeedin柏思薈、Condense Capital墾拓資本和ACRODiagnostics百斯醫(yī)學(xué)等。