Biotinylated Human Siglec-7 / CD328 Protein, His,Avitag? (MALS & SPR verified)

分子別名（Synonym）

CDw328,D-siglec,A79 membrane protein,p75,Adhesion inhibitory receptor molecule 1, AIRM-1

表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

Biotinylated Human Siglec-7, His,Avitag (SG7-H82E7) is expressed from human 293 cells (HEK293). It contains AA Gln 19 - Leu 353 (Accession # Q9Y286-1).

Predicted N-terminus: Gln 19

蛋白結(jié)構(gòu)（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag?).

The protein has a calculated MW of 40.5 kDa. The protein migrates as 55-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

標(biāo)記（Biotinylation）

Biotinylation of this product is performed using Avitag? technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白標(biāo)記度（Biotin:Protein Ratio)

Passed as determined by the HABA assay / binding ELISA.

內(nèi)毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

純度（Purity）

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制劑（Formulation）

Lyophilized from 0.22 μm filtered solution in 25 mM MES, 150 mM NaCl, pH5.5. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重構(gòu)方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲(chǔ)（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

Siglec-7是人類CD33相關(guān)Siglec受體的成員。Siglec-7的細(xì)胞外區(qū)域以一個(gè)可以結(jié)合唾液酸的N端V集Ig結(jié)構(gòu)域和兩個(gè)C2集Ig結(jié)構(gòu)區(qū)為特征。Siglec-7的細(xì)胞質(zhì)尾部有一個(gè)免疫受體酪氨酸基抑制基序（ITIM）和一個(gè)ITIM樣基序。Siglec-7被認(rèn)為是一種具有抑制潛力的唾液酸依賴性免疫受體，主要在人NK細(xì)胞、單核細(xì)胞和一小部分CD8+T細(xì)胞上表達(dá)。