Human ZNRF3 protein, His Tag (MALS verified)
分子別名（Synonym）
ZNRF3, Zinc,RING finger protein 3, RING-type E3 ubiquitin transferase ZNRF3, RING finger protein 203, KIAA1133, RNF203
表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）
Human ZNRF3, His Tag (ZN3-H52H3) is expressed from human 293 cells (HEK293). It contains AA Lys 56 - Met 219 (Accession # Q9ULT6-1).
Predicted N-terminus: Lys 56
蛋白結(jié)構(gòu)（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 19.7 kDa. The protein migrates as 22-25 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
內(nèi)毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
純度（Purity）
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制劑（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
重構(gòu)方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存儲（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
背景（Background）
跨膜E3泛素連接酶鋅環(huán)指3（ZNRF3）是Wnt信號傳導(dǎo)的負(fù)反饋調(diào)節(jié)因子。ZNRF3與Wnt受體復(fù)合物有關(guān)，通過促進(jìn)卷曲蛋白和LRP6的轉(zhuǎn)換來抑制Wnt信號傳導(dǎo)，并通過抑制Wnt信令通路在腸道干細(xì)胞區(qū)發(fā)揮腫瘤抑制作用。
關(guān)鍵字： ZNRF3;ZNRF3蛋白;ZNRF3重組蛋白;ACRO;百普賽斯;
百普賽斯集團(tuán)ACROBiosystems Group（股票代碼：301080）是成立于2010年的跨國生物科技公司，是為全球生物醫(yī)藥、健康產(chǎn)業(yè)領(lǐng)域提供關(guān)鍵生物試劑產(chǎn)品及解決方案的行業(yè)平臺型基石企業(yè)。2021年在創(chuàng)業(yè)板上市。百普賽斯集團(tuán)業(yè)務(wù)遍布全球，橫跨亞洲、北美洲、歐洲，在中國、美國、瑞士等12個城市設(shè)有辦公室、研發(fā)中心及生產(chǎn)基地。目前累計服務(wù)客戶超6000家，與全球Top 20醫(yī)藥企業(yè)均建立了長期、穩(wěn)定的合作伙伴關(guān)系。集團(tuán)旗下?lián)碛衅放艫CROBiosystems百普賽斯、bioSeedin柏思薈、Condense Capital墾拓資本和ACRODiagnostics百斯醫(yī)學(xué)等。