Monkeypox virus (strain Zaire-96-I-16) A35R Protein, His Tag

分子別名（Synonym）

A35R

表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

Monkeypox virus (strain Zaire-96-I-16) A35R , His Tag (A3R-M52H3) is expressed from human 293 cells (HEK293). It contains AA Arg 58 - Thr 181 (Accession # Q8V4U4).

Predicted N-terminus: Arg 58

蛋白結(jié)構(gòu)（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 15.5 kDa. The protein migrates as 15 kDa and 17 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

內(nèi)毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

純度（Purity）

>90% as determined by SDS-PAGE.

制劑（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重構(gòu)方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

猴痘是由猴痘病毒引起的一種罕見的人畜共患病，猴痘病毒已成為最嚴(yán)重的正痘病毒，由復(fù)雜的雙鏈DNA組成。這些病例大多發(fā)生在中非和西非。猴痘的發(fā)病機(jī)制是病毒從呼吸道粘膜侵入人體，在淋巴細(xì)胞中繁殖，并進(jìn)入血液，產(chǎn)生短暫的性病毒血癥。病毒在細(xì)胞中繁殖后，細(xì)胞可以侵入血液并傳播到全身皮膚，引起病變。EV表面的包膜糖蛋白A35R被預(yù)測會影響病毒顆粒的細(xì)胞間擴(kuò)散。