Human FOLR2 Protein, His Tag

分子別名（Synonym）

FOLR2,BETA-HFR,FBP,PL-1,FR-BETA,FR-P3,FBP

表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

Human FOLR2, His Tag (FO2-H5223) is expressed from human 293 cells (HEK293). It contains AA Thr 17 - His 228 (Accession # P14207-1).

Predicted N-terminus: Thr 17

蛋白結(jié)構(gòu)（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 26.5 kDa. The protein migrates as 30-35 kDa under reducing (R) condition (SDS-PAGE) due to N-linked glycosylation which has been verified by PNGase F digestion.

內(nèi)毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

純度（Purity）

>90% as determined by SDS-PAGE.

制劑（Formulation）

Lyophilized from 0.22 μm filtered solution in Tris and Glycine, pH7.5 with trehalose as protectant.

Contact us for customized product form or formulation.

重構(gòu)方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲(chǔ)（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

葉酸受體β也稱為葉酸受體2、FBP、FOLR2、beta-HFR、FBP/PL-1、FR-beta、FR-P3，是葉酸受體（FOLR）家族的成員。并介導(dǎo)5-甲基四氫葉酸向細(xì)胞內(nèi)部的遞送。該蛋白分別與FOLR1和FOLR3蛋白具有68%和79%的序列同源性。FOLR2蛋白最初被認(rèn)為只存在于胎盤中，但也在脾臟、骨髓和胸腺中檢測(cè)到。FOLR2主要在胎盤、中性粒細(xì)胞譜系細(xì)胞和一些CD34+造血祖細(xì)胞中表達(dá)。它在髓系白血病、頭頸部鱗狀細(xì)胞癌和幾種非上皮性癌癥中上調(diào)。它也在慢性炎癥部位的巨噬細(xì)胞和單核細(xì)胞上上調(diào)，包括類風(fēng)濕性關(guān)節(jié)炎滑膜和膠質(zhì)母細(xì)胞瘤。FOLR2是M-CSF存在下產(chǎn)生的巨噬細(xì)胞的標(biāo)志物，但不是GM-CSF。其表達(dá)與葉酸攝取能力的增強(qiáng)有關(guān)。治療藥物的葉酸偶聯(lián)物是靶向腫瘤相關(guān)巨噬細(xì)胞的潛在免疫治療工具。