Biotinylated Human ALPG / ALPPL2 Protein, His,Avitag? (MALS verified)
分子別名（Synonym）
ALPG,GCAP,ALP-1,ALPPL2,PLAP-like,ALPPL,Alkaline phosphatase, germ cell type
表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）
Biotinylated Human ALPG Protein, His,Avitag (ALG-H52E1) is expressed from human 293 cells (HEK293). It contains AA Ile 20 - Asp 503 (Accession # P10696-1).
Predicted N-terminus: Ile 20
蛋白結(jié)構(gòu)（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag?).
The protein has a calculated MW of 56.2 kDa. The protein migrates as 60-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
標(biāo)記（Labeling）
Biotinylation of this product is performed using Avitag? technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
蛋白標(biāo)記度（Protein Ratio）
Passed as determined by the HABA assay / binding ELISA.
內(nèi)毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
純度（Purity）
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制劑（Formulation）
Lyophilized from 0.22 μm filtered solution in 50 mM Tris,150 mM NaCl,pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
重構(gòu)方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存儲（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
背景（Background）
ALPPL2在幼稚多能干細(xì)胞中特異性高表達(dá)。此外，在幼稚狀態(tài)體細(xì)胞重編程、幼稚啟動轉(zhuǎn)化和其他系統(tǒng)中，使用ALPPL2啟動子驅(qū)動的熒光報告系統(tǒng)可以特異性地指示幼稚狀態(tài)多能性的建立或撤銷，從而將ALPPL2鑒定為幼稚狀態(tài)多能干性。特定的細(xì)胞表面標(biāo)記。一些研究發(fā)現(xiàn)，敲除ALPPL2可以特異性地影響幼稚狀態(tài)多能性的建立、穩(wěn)定性和維持，證明ALPPL2蛋白是一種功能性的幼稚狀態(tài)表面分子標(biāo)記。
關(guān)鍵字： ALPG;ALPG蛋白;ALPG重組蛋白;ACRO;百普賽斯;
百普賽斯集團(tuán)ACROBiosystems Group（股票代碼：301080）是成立于2010年的跨國生物科技公司，是為全球生物醫(yī)藥、健康產(chǎn)業(yè)領(lǐng)域提供關(guān)鍵生物試劑產(chǎn)品及解決方案的行業(yè)平臺型基石企業(yè)。2021年在創(chuàng)業(yè)板上市。百普賽斯集團(tuán)業(yè)務(wù)遍布全球，橫跨亞洲、北美洲、歐洲，在中國、美國、瑞士等12個城市設(shè)有辦公室、研發(fā)中心及生產(chǎn)基地。目前累計服務(wù)客戶超6000家，與全球Top 20醫(yī)藥企業(yè)均建立了長期、穩(wěn)定的合作伙伴關(guān)系。集團(tuán)旗下?lián)碛衅放艫CROBiosystems百普賽斯、bioSeedin柏思薈、Condense Capital墾拓資本和ACRODiagnostics百斯醫(yī)學(xué)等。