Human CD30 / TNFRSF8 Protein, His Tag

分子別名（Synonym）

TNFRSF8,CD30,D1S166E,Ki-1

表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

Human CD30, His Tag (CD0-H52H5) is expressed from human 293 cells (HEK293). It contains AA Phe 19 - Lys 379 (Accession # NP_001234.2).

Predicted N-terminus: Phe 19

蛋白結(jié)構(gòu)（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 40.4 kDa. The protein migrates as 53-80 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

內(nèi)毒素（Endotoxin）

Less than 0.1 EU per μg by the LAL method.

純度（Purity）

>90% as determined by SDS-PAGE.

制劑（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重構(gòu)方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

人CD30也稱為TNFRSF8，是腫瘤壞死因子受體家族的細(xì)胞膜蛋白和腫瘤標(biāo)志物。TNFRSF-8由活化的T細(xì)胞和B細(xì)胞表達(dá)，但不由靜息的T細(xì)胞或B細(xì)胞表達(dá)。此外，CD30與CD15一起在經(jīng)典霍奇金淋巴瘤細(xì)胞上表達(dá)。CD30是TNFSF8/CD30L的受體。CD30可以與TRAF2和TRAF5相互作用，介導(dǎo)導(dǎo)致NF-kappa-B激活的信號轉(zhuǎn)導(dǎo)。TNFRSF8可能在調(diào)節(jié)細(xì)胞生長和活化淋巴母細(xì)胞的轉(zhuǎn)化中發(fā)揮作用。TNFRSF8是凋亡的積極調(diào)節(jié)因子，也被證明可以限制自身反應(yīng)性CD8效應(yīng)T細(xì)胞的增殖潛力，保護(hù)身體免受自身免疫。