Human B7-H2 / ICOSLG Protein, His Tag

分子別名（Synonym）

ICOSLG,B7-H2,B7H2,B7RP-1,B7RP1,CD275,GL50,ICOS-L,ICOSL,LICOS,ICOS ligand

表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

Human B7-H2, His Tag (B72-H5221) is expressed from human 293 cells (HEK293). It contains AA Asp 19 - Ser 258 (Accession # NP_056074).

Predicted N-terminus: Asp 19

蛋白結(jié)構(gòu)（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 27.8 kDa. The protein migrates as 45-67 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

內(nèi)毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

純度（Purity）

>95% as determined by SDS-PAGE.

制劑（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重構(gòu)方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲(chǔ)（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

ICOS配體（ICOSLG）也稱為B7同源物2（B7-H2）、B7相關(guān)蛋白1（B7RP-1）和CD抗原CD275，屬于免疫球蛋白超家族和BTN/MOG家族。ICOSLG包含一個(gè)Ig-like C2型（免疫球蛋白樣）結(jié)構(gòu)域和一個(gè)Ig-like V型（免疫蛋白樣）功能域。異構(gòu)體1廣泛表達(dá)，而異構(gòu)體2僅在淋巴結(jié)、白細(xì)胞和脾臟中檢測(cè)到。B7-H2是T細(xì)胞特異性細(xì)胞表面受體ICOS的配體。B7-H2作為T細(xì)胞增殖和細(xì)胞因子分泌的共刺激信號(hào)，也誘導(dǎo)B細(xì)胞增殖和分化為漿細(xì)胞。B7-H2可能在介導(dǎo)局部組織對(duì)炎癥條件的反應(yīng)以及通過共刺激記憶T細(xì)胞功能調(diào)節(jié)二次免疫反應(yīng)中發(fā)揮重要作用。