Human CD209 / DC-SIGN Protein, His Tag

分子別名（Synonym）

DC-SIGN,CD209,DC-SIGN1,CLEC4L

表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

Human CD209, His Tag (CD9-H5246) is expressed from human 293 cells (HEK293). It contains AA Gln 59 - Ala 404 (Accession # Q9NNX6-1).

Predicted N-terminus: His

蛋白結(jié)構(gòu)（Molecular Characterization）

This protein carries a polyhistidine tag at the N-terminus.

The protein has a calculated MW of 41.3 kDa. The protein migrates as 40-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

內(nèi)毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

純度（Purity）

>90% as determined by SDS-PAGE.

制劑（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重構(gòu)方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲(chǔ)（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

CD209也被稱為CLEC4L、DC-SIGN和CD209抗原。病原體識(shí)別受體在未成熟樹突狀細(xì)胞（DC）表面表達(dá)，參與初級(jí)免疫反應(yīng)的啟動(dòng)。在DC上，它是ICAM2和ICAM3的高親和力受體，通過與甘露糖樣碳水化合物結(jié)合?？勺鳛?/span>DC滾動(dòng)受體，通過結(jié)合內(nèi)皮ICAM2介導(dǎo)DC前體從血液到組織的跨內(nèi)皮遷移。似乎通過在DC和T細(xì)胞之間形成的免疫突觸中與T細(xì)胞上的ICAM3結(jié)合來調(diào)節(jié)DC誘導(dǎo)的T細(xì)胞增殖。