Biotinylated Human LILRB4 / CD85k / ILT3 Protein, His,Avitag? (MALS verified)

分子別名（Synonym）

LILRB4,ILT3,LIR5,CD85K,HM18

表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

Biotinylated Human LILRB4, His,Avitag (LI4-H82E4) is expressed from human 293 cells (HEK293). It contains AA Gln 22 - Glu 259 (Accession # AAH26309.1).

Predicted N-terminus: Gln 22

蛋白結(jié)構(gòu)（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag?).

The protein has a calculated MW of 29.8 kDa. The protein migrates as 32-40 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

標(biāo)記（Labeling）

Biotinylation of this product is performed using Avitag? technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白標(biāo)記度（Protein Ratio）

Passed as determined by the HABA assay / binding ELISA.

內(nèi)毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

純度（Purity）

>90% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制劑（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重構(gòu)方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲(chǔ)（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

白細(xì)胞免疫球蛋白樣受體亞家族B成員4（LILRB4）也稱(chēng)為CD85抗原樣家族成員K（CD85K）、免疫球蛋白類(lèi)轉(zhuǎn)錄物3（ILT-3）、白細(xì)胞免疫珠蛋白樣受體5（LIR-5）、單核細(xì)胞抑制受體HM18，屬于白細(xì)胞免疫標(biāo)球蛋白樣接收器（LIR）家族。LILRB4/CD85K含有2個(gè)Ig樣C2型（免疫球蛋白樣）結(jié)構(gòu)域。在單核細(xì)胞、巨噬細(xì)胞、樹(shù)突狀細(xì)胞、肺、自然殺傷細(xì)胞和B細(xì)胞中檢測(cè)到CD85K。LILRB4/CD85K是I類(lèi)MHC抗原的受體。CD85K識(shí)別廣泛的HLA-a、HLA-B、HLA-C和HLA-G等位基因，參與免疫反應(yīng)的下調(diào)和耐受的發(fā)展。LILRB4干擾TNFRSF5信號(hào)傳導(dǎo)和NF-kappa-B上調(diào)，并抑制受體介導(dǎo)的細(xì)胞蛋白磷酸化和細(xì)胞內(nèi)鈣離子的動(dòng)員。