Human ASGR1 / ASGPR1 Protein, His Tag (MALS & SPR verified)

分子別名（Synonym）

ASGP-R 1, ASGPR 1, C-type lectin domain family 4 member H1, Hepatic lectin H1, HL-1

表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

Human ASGR1 Protein, His Tag (AS1-H5243) is expressed from human 293 cells (HEK293). It contains AA Gln 62 - Leu 291 (Accession # P07306-1).

Predicted N-terminus: His

蛋白結(jié)構(gòu)（Molecular Characterization）

This protein carries a polyhistidine tag at the N-terminus.

The protein has a calculated MW of 28.3 kDa. The protein migrates as 36-45 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

內(nèi)毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

純度（Purity）

>90% as determined by SDS-PAGE.

制劑（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, 0.5M Arginine, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重構(gòu)方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

ASGPR是一種跨膜C型凝集素，可識別多種含有末端半乳糖（Gal）或N-乙酰半乳糖胺（GalNAc）殘基的配體，已被鑒定為肝細(xì)胞上的高選擇性受體。ASGR由主要亞基（ASGR1）和次要亞基（ASDR2）組成。ASGR1已被證明能有效靶向質(zhì)膜，并經(jīng)歷組成性內(nèi)吞和再循環(huán)，主要在人類肝臟中表達(dá)。