Cynomolgus Siglec-3 / CD33 Protein, His Tag

分子別名（Synonym）

CD33,SIGLEC3,gp67

表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

Cynomolgus Siglec-3, His Tag (CD3-C52H4) is expressed from human 293 cells (HEK293). It contains AA Met 16 - Leu 228 (Accession # XP_045235686.1).

Predicted N-terminus: Met 16

蛋白結(jié)構(gòu)（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 25.7 kDa. The protein migrates as 38-43 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

內(nèi)毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

純度（Purity）

>90% as determined by SDS-PAGE.

制劑（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重構(gòu)方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲(chǔ)（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

骨髓細(xì)胞表面抗原CD33也被稱為SIGLEC3、Siglecs（唾液酸結(jié)合Ig樣凝集素）和GP67，是一種單通道I型膜蛋白，屬于免疫球蛋白超家族和SIGLEC（唾液酸連接Ig類凝集素）家族。人CD33/Siglec-3 cD編碼一個(gè)364個(gè)氨基酸（aa）的多肽，具有疏水信號(hào)肽、N端Ig樣V型結(jié)構(gòu)域、一個(gè)Ig樣C2型結(jié)構(gòu)域，一個(gè)跨膜區(qū)和一個(gè)細(xì)胞質(zhì)尾部。CD33/Siglec-3通常被認(rèn)為是髓系特異性的，但也可以在一些淋巴細(xì)胞上發(fā)現(xiàn)。在免疫反應(yīng)中，CD33/Siglec-3可能通過(guò)其SH2結(jié)構(gòu)域招募細(xì)胞質(zhì)磷酸酶，阻斷信號(hào)分子去磷酸化的信號(hào)轉(zhuǎn)導(dǎo)，從而對(duì)配體誘導(dǎo)的酪氨酸磷酸化起到抑制受體的作用。CD33/Siglec-3誘導(dǎo)急性髓系白血病細(xì)胞凋亡。