Human GUCY2C / Guanylyl cyclase C Protein, His Tag (MALS verified)

分子別名（Synonym）

GUCY2C,GUC2C,STAR,STA receptor,hSTAR,GC-C

表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

Human GUCY2C, His Tag (GUC-H52H5) is expressed from human 293 cells (HEK293). It contains AA Ser 24 - Gln 430 (Accession # P25092-1).

Predicted N-terminus: Ser 24

蛋白結(jié)構(gòu)（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 47.9 kDa. The protein migrates as 55-85 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

內(nèi)毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

純度（Purity）

>90% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制劑（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重構(gòu)方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲(chǔ)（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

GUCY2C（Guanylyl Cyclase C），也稱為熱穩(wěn)定腸毒素受體，是鳥苷酸環(huán)合酶（gc）家族的I型跨膜蛋白，通過產(chǎn)生cGMP發(fā)出信號(hào)。鳥苷酸環(huán)化酶C（GUCY2C）及其激素鳥苷酸和尿鳥苷酸最近成為一個(gè)旁分泌軸，保護(hù)腸粘膜完整性免受突變、化學(xué)和炎癥損傷。GUCY2C小鼠CAR-T細(xì)胞識(shí)別并殺死內(nèi)源性表達(dá)GUCY2C的人結(jié)直腸癌癌癥細(xì)胞。因此，我們已經(jīng)確定了一種人類GUCY2C-特異性CAR-T細(xì)胞治療方法，該方法可能被開發(fā)用于治療GUCY2C外轉(zhuǎn)移性癌癥。