Human IL-2 R alpha / CD25 Protein, His Tag

分子別名（Synonym）

IL2RA,CD25,p55,IL2-RA,IL-2-RA

表達區(qū)間及表達系統(tǒng)（Source）

Human IL-2 R alpha, His Tag (ILA-H52H9) is expressed from human 293 cells (HEK293). It contains AA Glu 22 - Cys 213 (Accession # NP_000408).

Predicted N-terminus: Glu 22

蛋白結(jié)構(gòu)（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 23.6 kDa. The protein migrates as 34-46 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

內(nèi)毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

純度（Purity）

>95% as determined by SDS-PAGE.

制劑（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重構(gòu)方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

白細胞介素-2受體亞基α（IL2RA）也稱為IL-2R亞基α、IL-2-RA、IL2-RA、TAC抗原、p55、CD抗原CD25，是一種I型跨膜糖蛋白。IL2RA在活化的T細胞和調(diào)節(jié)性T細胞上表達，并且能夠自身以低親和力結(jié)合IL2。然而，當(dāng)IL2RA與IL2受體β鏈和γ鏈非共價結(jié)合，隨后啟動MAPK或JAK/STAT等細胞內(nèi)信號通路時，會產(chǎn)生配體誘導(dǎo)的高親和力異三聚體受體復(fù)合物。在樹突狀細胞（DC）上，CD25以前被認為是一種激活標志物，而小鼠和人類DC都可以表達CD25，但它們不表達IL-2受體的β鏈，這是執(zhí)行IL-2信號傳導(dǎo)所必需的。