Human CA125 / MUC16 (13810-14451) Protein, His Tag (MALS verified)

分子別名（Synonym）

CA125,CA-125,CA125MUC-16,FLJ14303,MUC16,mucin 16,mucin-16

表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

Human CA125 (13810-14451), His Tag (CA5-H52H6) is expressed from human 293 cells (HEK293). It contains AA Pro 13810 - Pro 14451 (Accession # Q8WXI7-1).

Predicted N-terminus: Pro 13810

蛋白結(jié)構(gòu)（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 73.7 kDa. The protein migrates as 90-130 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

內(nèi)毒素（Endotoxin）

Less than 0.1 EU per μg by the LAL method.

純度（Purity）

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制劑（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重構(gòu)方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲(chǔ)（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

CA125，也被稱為MUC16，是一種粘蛋白，可在I型跨膜或分泌形式中發(fā)現(xiàn)，用于監(jiān)測(cè)上皮性卵巢癌癥治療的進(jìn)展。CA 125分子幾乎可以肯定是一種以O-鍵為主的糖蛋白。它在大小和電荷方面都是異質(zhì)的，很可能是由于側(cè)鏈在體液中的持續(xù)去糖基化。在自然條件下，它以非常大的復(fù)合體（可能高達(dá)400萬(wàn)道爾頓）的形式存在。