Human IL-4 Protein, His Tag (MALS verified)

分子別名（Synonym）

IL-4,BCGF1,BSF1

表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

Human IL-4, His Tag (IL4-H52H9) is expressed from human 293 cells (HEK293). It contains AA His 25 - Ser 153 (Accession # P05112-1).

Predicted N-terminus: His 25

蛋白結(jié)構(gòu)（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 16.9 kDa. The protein migrates as 22-24 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

內(nèi)毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

純度（Purity）

>90% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制劑（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重構(gòu)方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲(chǔ)（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

白細(xì)胞介素4是一種誘導(dǎo)幼稚輔助T細(xì)胞（Th0細(xì)胞向Th2細(xì)胞）分化的細(xì)胞因子。在IL-4和IL-13的存在下，Th1型反應(yīng)中產(chǎn)生的細(xì)胞因子，特別是在過(guò)敏和寄生蟲(chóng)感染期間，巨噬細(xì)胞會(huì)被不同程度地激活，這種細(xì)胞因子是白細(xì)胞介素4受體的配體。白細(xì)胞介素4受體也與IL13結(jié)合，這可能導(dǎo)致該細(xì)胞因子和IL13的許多重疊功能。STAT6是一種信號(hào)轉(zhuǎn)導(dǎo)子和轉(zhuǎn)錄激活子，已被證明在介導(dǎo)這種細(xì)胞因子的免疫調(diào)節(jié)信號(hào)中起著核心作用。最近，研究人員發(fā)現(xiàn)，細(xì)胞因子IL-4在幾種基因缺陷小鼠品系（包括Itk、KLF2、CBP和Id3）胸腺中先天CD8+T細(xì)胞的發(fā)育中起著關(guān)鍵作用，這些小鼠品系以前沒(méi)有接觸過(guò)抗原。